|Table of Contents|

Total flavonoids of verbena officinalis L.regulate autophagy and inhibit hepatocellular carcinoma cell proliferation through AKT/mTOR pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 14
Page:
2631-2638
Research Field:
Publishing date:

Info

Title:
Total flavonoids of verbena officinalis L.regulate autophagy and inhibit hepatocellular carcinoma cell proliferation through AKT/mTOR pathway
Author(s):
YANG Jinxu; SUN Zihong; XU Lin
Department of Nursing,Luohe Medical College,Henan Luohe 462002,China.
Keywords:
total flavonoids of verbena officinalis L.; AKT/mTOR pathway; autophagy; hepatocellular carcinoma; cell proliferation
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2023.14.011
Abstract:
Objective:To analyze the effect of total flavonoids of verbena officinalis L.(TFV) on autophagy and proliferation of hepatocarcinoma cells,and to explore its mechanism.Methods:MDC staining method was used to detect the level of autophagy vesicles.Western blotting was used to detect autophagy-related protein levels.3-MA and Rapamycin（RAPA）were used to study the effects of TFV on autophagy and cell proliferation,and CCK-8 method was used to detect cell survival.The tumor formation experiment in nude mice verified the effects of TFV on tumor weight,tumor volume and autophagy-related protein levels in nude mice.Results:TFV could increase the fluorescent particles labeled by MDC staining in HepG2 and Huh-7 cells,and the fluorescent particles significantly increased with the increase of concentration.It could promote the expression of autophagy protein Beclin-1 and LC3 II/LC3 I in HepG2 and Huh-7 cells,and reduce the levels of p-AKT/AKT and p-mTOR/mTOR (P＜0.05).Compared with the TFV group,the inhibition rate and LC3 II/LC3 I levels in the TFV+3-MA group were significantly reduced,and the p-mTOR/mTOR levels was significantly increased (P＜0.05).Compared with the TFV group,the inhibition rate and LC3 II/LC3 I levels in the TFV+RAPA group were significantly increased,and the levels of p-mTOR/mTOR were significantly reduced (P＜0.05).Compared with the TFV group,LC3 II/LC3 I levels of the tumor bodies of nude mice in the TFV+3-MA group decreased significantly,and tumor mass,tumor volume,and p-mTOR/mTOR increased significantly (P＜0.05).Conclusion:TFV can inhibit the proliferation of hepatocellular carcinoma cells by inducing autophagy by inhibiting the AKT/mTOR signaling pathway.

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