|Table of Contents|

lncRNA DLEU2/miR-455-3p/OTUD7B axis promotes the malignant progression of cervical carcinoma by regulating PI3K/AKT signaling pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 14
Page:
2614-2623
Research Field:
Publishing date:

Info

Title:
lncRNA DLEU2/miR-455-3p/OTUD7B axis promotes the malignant progression of cervical carcinoma by regulating PI3K/AKT signaling pathway
Author(s):
FENG Yuling1; GU Yannan1; LING Jianmei1; DING Yiqian2
1.Department of Obstetrics and Gynecology;2.Department of Gynecology,Nantong Maternal and Child Health Hospital,Jiangsu Nantong 226000,China.
Keywords:
cervical carcinoma; DLEU2; miR-455-3p; OTUD7B; PI3K/AKT signaling pathway
PACS:
R737.33
DOI:
10.3969/j.issn.1672-4992.2023.14.009
Abstract:
Objective:To investigate the effects of long non-coding RNA DLEU2 (lncRNA DLEU2,DLEU2) on the malignant progression of cervical carcinoma.Methods:TCGA database and qRT-PCR were used to analyze DLEU2 expression in cervical carcinoma tissues and cell lines.The effects of shRNA-DLEU2 (sh-DLEU2) on cell proliferation,migration,invasion,and apoptosis of cervical carcinoma were assessed using CCK-8,Western blotting,immunofluorescence,wound healing,Transwell,and TUNEL assays.Bioinformatics and dual-luciferase reporter assays were conducted to identify the target genes of DLEU2 and miR-455-3p,and the effects of miR-455-3p inhibitor/mimic on cell growth of cervical carcinoma and PI3K/AKT signaling pathway were examined.The effect of sh-DLEU2 on tumor growth in vivo was tested by tumor-bearing experiment in nude mice.Results:DLEU2 expression in cervical carcinoma tissues and cell lines was significantly up-regulated (P＜0.01).sh-DLEU2 inhibited the proliferation,migration,invasion (P＜0.01),and promoted apoptosis (P＜0.001) of Hela and SiHa cells.It was confirmed that DLEU2 combined with miR-455-3p.OTUD7B acted as a target gene for miR-455-3p.miR-455-3p inhibitor promoted cell malignant development and activated PI3K/AKT signaling pathway,whereas sh-DLEU2 reversed its impacts (P＜0.01).miR-455-3p mimic also partially reversed the effects of Oe-OTUD7B on cells (P＜0.01).Furthermore,sh-DLEU2 inhibited the growth of tumor-bearing tissue in nude mice (P＜0.01).Conclusion:DLEU2 activates PI3K/AKT signaling pathway through miR-455-3p/OTUD7B axis to promote the malignant development of cervical carcinoma.

References:

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