|Table of Contents|

Overexpression of lncRNA LINC00886 affects the biological behavior of breast cancer MDA-MB-231 cells by targeting miR-451a

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 14
Page:
2593-2600
Research Field:
Publishing date:

Info

Title:
Overexpression of lncRNA LINC00886 affects the biological behavior of breast cancer MDA-MB-231 cells by targeting miR-451a
Author(s):
GAO Jianchao; ZHANG Jingli; ZHANG Zhisheng; LI Xiaoxia; MA Ke; FENG Zhilin; ZHOU Haifeng; WANG Zhanhai; LIANG Wanping
Department of Breast Surgery,the First Hospital Affiliated to Hebei North University,Hebei Zhangjiakou 075000,China.
Keywords:
long non-coding RNA LINC00886; microRNA-451a; breast cancer MDA-MB-231 cells; biological behavior
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2023.14.006
Abstract:
Objective:To explore the effects of overexpression of long non-coding RNA (lncRNA) LINC00886 on the biological behavior of breast cancer (BC) MDA-MB-231 cells by regulating microRNA-451a (miR-451a).Methods:Fluorescence quantitative PCR (qRT-PCR) was used to measure the expression of lncRNA LINC00886 and miR-451a in human breast epithelial cell line (MCF-12A) and four BC cell lines (HCC1937,SUM159,SK-BR-3 and MDA-MB-231).MDA-MB-231 cells were divided into control group,LINC00886-NC group,LINC00886 group,LINC00886+miR-NC group and LINC00886+miR-451a group.The expression levels of lncRNA LINC00886 and miR-451a in MDA-MB-231 cells were detected by qRT-PCR.The proliferation ability of MDA-MB-231 cells was detected by clone formation experiment and EdU staining.The apoptosis,invasion and migration of MDA-MB-231 cells were detected by flow cytometry,Transwell chamber assay and scratch healing assay,respectively.The protein expression levels of proliferating cell nuclear antigen (PCNA),Bcl-2 associated X protein (Bax),B cell lymphocytoma-2 (Bcl-2),activated cysteine aspartic protease 3 (Cleaved caspase-3),matrix metalloproteinase (MMP)-2,MMP-9 of MDA-MB-231 cells were detected by Western blot.The targeting relationship between lncRNA LINC00886 and miR-451a was detected by dual luciferase reporter gene.Results:Compared with human breast epithelial cells MCF-12A,the expression level of lncRNA LINC00886 in BC cell lines was significantly decreased,and the expression level of miR-451a was significantly increased (P＜0.05).The overexpression of lncRNA LINC00886 can increase the apoptosis rate of MDA-MB-231 cells,the expression of Bax and Cleaved caspase-3 protein,and reduce miR-451a expression,colony formation number,percentage of EdU positive cells,number of invasive cells,migration rate,protein expression of PCNA,Bcl-2,MMP-2,MMP-9 (P＜0.05).The up-regulation of miR-451a expression could attenuate the effect of overexpression of lncRNA LINC00886 on MDA-MB-231 cells (P＜0.05).lncRNA LINC00886 can target the expression of miR-451a.Conclusion:Overexpression of lncRNA LINC00886 can promote the apoptosis of MDA-MB-231 cells and inhibit the proliferation,invasion and migration of MDA-MB-231 cells by targeting the inhibition of miR-451a expression.

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Memo

Memo:
河北省医学科学研究课题计划项目（编号：20200569）；张家口市重点研发计划项目（编号：2121135D）
Last Update: 1900-01-01