|Table of Contents|

The study of miR-34s enhancing the radiosensitivity of colorectal cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 14
Page:
2588-2592
Research Field:
Publishing date:

Info

Title:
The study of miR-34s enhancing the radiosensitivity of colorectal cancer cells
Author(s):
CHEN Shuangjing1; GAO Qichen1; ZENG Hao1; WANG Jun2; ZHU Zheng1; LIU Bo1; SU Pu1
1.Research Department;2.Pharmacy Department,PLA Rocket Force Characteristic Medical Center,Beijing 100088,China.
Keywords:
colorectal cancer; radiosensitivity; miR-34s; DNA double strand breaks
PACS:
R735.3
DOI:
10.3969/j.issn.1672-4992.2023.14.005
Abstract:
Objective:To investigate the effects of miR-34s on the radiosensitivity of colorectal cancer (CRC) cells and the role of miR-34s in ionizing radiation-induced DNA damage.Methods:Real-time quantitative fluorescence PCR (qRT-PCR) was used to detect the expression of miR-34a/b/c-5p in CRC cells and the expression trend within 24 h after ionizing radiation.Cell survival curves were established based on clonogenic assay and multitarget/single-hit model to analyze the effects of miR-34a/b/c-5p on the radiosensitivity of CRC cells.Cells overexpressing miR-34a/b/c-5p were exposed to ionizing radiation,and γH2AX foci were detected using immunofluorescence assay after irradiation to analyze the effects of miR-34a/b/c-5p on ionizing radiation-induced DNA damage.Results:The expression levels of miR-34a/b/c-5p in HCT116 cells were significantly higher than that in HT29 cells (P＜0.05).Within 24 h after 4 Gy irradiation,miR-34a/b/c-5p showed a bimodal expression pattern in HCT116 cells.Compared with the miR-NC group,overexpression of miR-34a/b/c-5p could significantly enhance the radiosensitivity of CRC cells.The mean lethal dose (D0) and quasi-threshold dose (Dq) were significantly decreased in the miR-34a/b/c-5p group,while the sensitizing enhancement ratio (SER) was significantly increased.Overexpression of miR-34a/b/c-5p significantly increased the level of ionizing radiation-induced DNA double strand breaks (DSBs),and the number of γH2AX foci in miR-34a/b/c-5p group were significantly higher than that in miR-NC group at 1 h and 8 h after irradiation (P＜0.05).Conclusion:miR-34s are radioresponsive miRNA molecules,and overexpression of miR-34s can increase the level of ionizing radiation-induced DNA damage and enhance the radiosensitivity of CRC cells.

References:

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