|Table of Contents|

The effect of circ_0001721/miR-198 on the biological behavior of prostate cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 14
Page:
2575-2580
Research Field:
Publishing date:

Info

Title:
The effect of circ_0001721/miR-198 on the biological behavior of prostate cancer cells
Author(s):
WANG Hongmei1; XIE Bin2; CHEN Hanchao1; GUAN Hong1; LI Chunyun1
1.Department of Laboratory Medicine;2.Department of Critical Care Medicine,Haikou Affiliated Hospital of Central South University Xiangya School of Medicine,Hainan Haikou 570203,China.
Keywords:
prostate cancer; circ_0001721; miR-198; proliferation; migration; invasion; apoptosis
PACS:
R737.25
DOI:
10.3969/j.issn.1672-4992.2023.14.003
Abstract:
Objective:To determine the impact of circ_0001721/miR-198 molecular axis on the biological behavior of human prostate cancer cells DU145 and its related mechanism.Methods:The expression levels of circ_0001721 and miR-198 were detected by qRT-PCR.DU145 cells were transfected with si-circ_0001721,miR-198 mimics,anti-miR-198 and their negative controls (si-NC,miR-NC,anti-miR-NC),respectively.Cell proliferation,cloning,apoptosis,migration and invasion were assessed using CCK-8,colony formation assay,flow cytometry and Transwell assay,respectively.Targeting between circ_0001721 and miR-198 was validated using dual-luciferase reporter assay.The protein expression of E-cadherin and N-cadherin was detected by Western blot.Results:Compared with normal tissue,circ_0001721 expression in prostate cancer tissue was increased (P＜0.05),while miR-198 expression in prostate cancer tissue was decreased (P＜0.05).Compared with transfection of si-NC or miR-NC,the cell proliferation inhibition rate,apoptosis rate and the protein level of E-cadherin were increased (P＜0.05),while the number of cell clone formation,migration,invasion and the protein level of N-cadherin were decreased (P＜0.05) after transfection of si-circ_0001721 or miR-198 mimics.circ_0001721 could target miR-198.Compared with co-transfection with si-circ_0001721 and anti-miR-NC,the cell proliferation inhibition rate,apoptosis rate and the protein level of E-cadherin were reduced (P＜0.05),while the number of cell clone formation,migration,invasion and the protein level of N-cadherin were increased (P＜0.05) after co-transfection with si-circ_0001721 and anti-miR-198.Conclusion:Inhibition of circ_0001721 expression could reduce prostate cancer cell growth and metastasis by up-regulating miR-198.

References:

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海南省卫生计生行业科研项目（编号：19A200164）
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