|Table of Contents|

MLN4924 enhances PD-L1 expression in human lung adenocarcinoma A549 cells by inhibiting the Neddylation pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 12
Page:
2223-2228
Research Field:
Publishing date:

Info

Title:
MLN4924 enhances PD-L1 expression in human lung adenocarcinoma A549 cells by inhibiting the Neddylation pathway
Author(s):
LONG Guo; LIU Dan; DENG Shukai
Department of Respiratory and Critical Care Medicine,Affiliated Hospital of Southwest Medical University,Sichuan Luzhou 646000,China.
Keywords:
lung cancer; A549 cells; MLN4924; programmed death-ligand 1(PD-L1); sex-determining region Y-box containing gene 2(SOX2)
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2023.12.009
Abstract:
Objective:To investigate the effect of MLN4924 on the expression of programmed death-ligand 1(PD-L1) in lung adenocarcinoma A549 cells and its possible underlying mechanism.Methods:Human lung adenocarcinoma A549 cells were cultured in vitro and treated with different doses of MLN4924 (0, 0.25, 0.5, 0.75, 1, 5, 10 μmol/L) for 24, 48 and 72 h.The inhibition rate of cell proliferation was detected by CCK-8 method. The median inhibitory concentration (IC50) of each time group was calculated.At the same time,the expression of PD-L1 was detected by flow cytometry.Meanwhile,the obvious up-regulation time of PD-L1 and its IC50 were selected for subsequent experiments.Follow-up experiments were divided into two groups:Blank control group and IC50 MLN4924 treatment group.After 72 hours of culture,the expression of FBXW2 protein,muscle segment homeobox2(MSX2) protein,sex-determining region Y-box containing gene 2(SOX2) and PD-L1 protein was detected by Western blotting method,and the expression of PD-L1 mRNA was detected by qPCR method.Results:The IC50 values at 24, 48 and 72 h were 1.976, 0.647, 0.469 μmol/L.Compared with the control group,the expression of PD-L1 in A549 cells treated with MLN4924 was up-regulated in a time and concentration dependent manner.Compared with the control group,the expressions of FBXW2 and SOX2 proteins in MLN4924 treatment group were decreased,while the expressions of MSX2 protein,and the expressions of the mRNA and protein of PD-L1 were increased.Conclusion:MLN4924 enhances the expression of PD-L1 in A549 cells by inhibiting Neddylation pathway.

References:

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