|Table of Contents|

Effects of RMI2 transcriptionally activated by TRIM28 on the malignant biological behaviors of hepatocellular carcinoma SK-Hep-1 cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 12
Page:
2216-2223
Research Field:
Publishing date:

Info

Title:
Effects of RMI2 transcriptionally activated by TRIM28 on the malignant biological behaviors of hepatocellular carcinoma SK-Hep-1 cells
Author(s):
WU Tianfang1; DAI Dong2; WANG Changyuan3
1.Department of Hepatobiliary Surgery,the People's Hospital of Qiannan,Guizhou Qiannan 558000,China;2.Department of Molecular Imaging and Nuclear Medicine,Tianjin Medical University Cancer Institute﹠Hospital,Tianjin 300000,China;3.Department of Hepatobiliary Surgery,Guizhou Provincial People's Hospital,Guizhou Guiyang 550000,China.
Keywords:
HCC; RMI2; TRIM28; cell cycle; cell apoptosis
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2023.12.008
Abstract:
Objective:To investigate the impacts of RecQ-mediated genome instability protein 2 (RMI2) on the aggressive phenotypes of hepatocellular carcinoma (HCC) SK-Hep-1 cells.Methods:GEPIA database detected RMI2 andtripartite motif 28 (TRIM28) expression in HCC tissues,predicted HCC tumor stages,overall survival rate or disease-free survival rate of HCC patients as well as the correlation between RMI2 and TRIM28 expression in HCC tissues.RT-qPCR and Western blot examined RMI2 and TRIM28 expression.The binding sequences between RMI2 promoter and TRIM28 were predicted based on HumanTFDB database.Luciferase reporter and ChIP assays substantiated the binding between RMI2 and TRIM28.Cell proliferation was judged by MTT and EdU staining.Flow cytometry analysis and TUNEL assay respectively measured cell cycle and apoptosis.Western blot analyzed the expression ofproliferation and apoptosis-associated factors.Results:RMI2 and TRIM28 expression were increased in HCC tissues and cells relative to normal tissues and liver epithelial cell line(P<0.001) and associated with poor prognosis.After RMI2 was knocked down,the proliferative ability of SK-Hep-1 cells was decreased (P<0.001).Cells were arrested at G0/G1 phase (P<0.001) and cell apoptotic rate was increased (P<0.001).RMI2 and TRIM28 expression had a positive correlation in HCC tissues and TRIM28 could up-regulate RMI2 expression (P<0.001) via binding with RMI2 promoter.TRIM28 overexpression reversed the impacts of RMI2 knockdown on SK-Hep-1 cell proliferation,cell cycle and apoptosis (P<0.05).Conclusion:RMI2 transcriptionally activated by TRIM28 might regulate the aggressive phenotypesof SK-Hep-1 cells.

References:

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Memo

Memo:
2023年度贵州省卫生健康委科学技术基金项目（编号：gzwkj2023-349）
Last Update: 1900-01-01