|Table of Contents|

Long non-coding RNA SNHG1 regulating proliferation,migration and invasion of esophageal cancer cells by targeting miR-340-5p/CCND1 axis

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 12
Page:
2209-2215
Research Field:
Publishing date:

Info

Title:
Long non-coding RNA SNHG1 regulating proliferation,migration and invasion of esophageal cancer cells by targeting miR-340-5p/CCND1 axis
Author(s):
HUANG Jianwei1; LI Yufeng1; ZHANG Quan1; WANG Lu1; LIU Xiaoyu1; ZENG Hai2
1.Department of Thoracic Surgery;2.Department of General Surgery,Hongqi Hospital Affiliated to Mudanjiang Medical College,Heilongjiang Mudanjiang 157011,China.
Keywords:
esophageal cancer; long non-coding RNA small nucleolar RNA host gene 1; miR-340-5p; cyclin 1; proliferation; migration; invasion
PACS:
R735.1
DOI:
10.3969/j.issn.1672-4992.2023.12.007
Abstract:
Objective:To analyze the influences of long non-coding RNA small nucleolar RNA host gene 1(LncRNA SNHG1) on the proliferation,migration and invasion of esophageal cancer cells by targeting miR-340-5p/cyclin 1(CCND1) axis.Methods:Human esophageal epithelial cells and esophageal cancer cells KYSE-30,TE-1,NEC,Eca109 were cultured in vitro.The levels of LncRNA SNHG1,miR-340-5p and CCND1 mRNA in cells were measured by qRT-PCR.NEC cells in logarithmic phase were divided into control group,sh NC1 group,sh LncRNA SNHG1 group,sh NC2 group,sh CCND1 group,sh LncRNA SNHG1+miR-340-5p inhibitor group,and sh CCND1+miR-340-5p inhibitor group.CCK-8 method was used to determine the cell proliferation ability.Transwell chamber method was used to measure the invasion and migration of cells.The protein expressions of CCND1,Ki-67 and MMP-2 were detected by Western blot.Double luciferase was applied to verify the targeting relationship between miR-340-5p and LncRNA SNHG1,CCND1,and the transfection reagent was injected into the tumor of nude mice for in vivo test.Results:Compared with human esophageal epithelial cells,the expression of LncRNA SNHG1 and CCND1 mRNA in the esophageal cancer cells KYSE-30,TE-1,NEC,and Eca109 increased,and the expression of miR-340-5p decreased (P<0.05),in which,NEC cells had the most obvious changes,so NEC cells were used as the strain for next study.Compared with the control group and sh NC group,the number of LncRNA SNHG1,OD450,the number of invading cells,the number of migrating cells,the expression of Ki-67 and MMP-2 in NEC cells in sh LncRNA SNHG1 group decreased (P<0.05).Compared with the control group and the sh NC group,the expression of CCND1 mRNA and protein,OD450,the number of invading cells,the number of migrating cells,the expression of Ki-67 and MMP-2 decreased in the sh CCND1 group (P<0.05).miR-340-5p bound to LncRNA SNHG1 and CCND1.Compared with sh LncRNA SNHG1 group,the OD450,the number of invading cells,the number of migrating cells,the expression of Ki-67 and MMP-2 proteins in sh LncRNA SNHG1+miR-340-5p inhibitor group increased (P<0.05).Compared with sh CCND1 group,the OD450,the number of invading cells,the number of migrating cells,Ki-67,MMP-2 proteins in sh CCND1+miR-340-5p inhibitor group increased (P<0.05).The nude mouse tumor transplantation experiment was carried out for in vivo verification.Conclusion:LncRNA SNHG1 silencing may inhibit the proliferation,invasion and migration of esophageal cancer NEC cells by regulating the expression of miR-340-5p/CCND1,which is also verified in nude mice.

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黑龙江省省属高等学校基本科研业务费科研项目（编号：2020-KYYWFMY-0032）
Last Update: 1900-01-01