|Table of Contents|

The down-regulation of ERp57 inhibits the proliferation,migration and invasion of esophageal cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 12
Page:
2186-2190
Research Field:
Publishing date:

Info

Title:
The down-regulation of ERp57 inhibits the proliferation,migration and invasion of esophageal cancer cells
Author(s):
NIU Yujie; GUO Cairu
Central Laboratory,Luoyang Central Hospital Affiliated to Zhengzhou University,Henan Luoyang 471009,China.
Keywords:
esophageal squamous cell carcinoma; ERp57; proliferation; migration; invasion
PACS:
R735.1
DOI:
10.3969/j.issn.1672-4992.2023.12.003
Abstract:
Objective:To investigate the expression of ERp57 in esophageal squamous cell carcinoma tissues and the effects of down-regulation of ERp57 on the biological function of TE-1 cells.Methods:The expression of ERp57 in esophageal cancer was analyzed by GEPIA.RT-qPCR and Western-blot detected the expression of ERp57 in esophageal cancer tissues and cell lines.After ERp57 of TE-1 cells was silenced by shRNA,the cell proliferation,apoptosis,migration and invasion were detected by CCK8,TUNEL,scratch and Transwell experiments.Results:The mRNA and protein expression levels of ERp57 in esophageal cancer tissues were higher than those in paracancerous tissues(P<0.05),and the mRNA and protein expression levels of ERp57 in esophageal cancer cell lines were higher than those in esophageal epithelial cells (P<0.05).Silencing ERp57 weakened the proliferation ability of TE-1 cells,increased the proportion of apoptosis,and weakened the migration and invasion of TE-1 cells.Conclusion:Silencing ERp57 expression can inhibit the proliferation,migration and invasion of esophageal cancer TE-1 cells,which can provide theoretical basis for esophageal cancer research.

References:

［1］ SUNG H,FERLAY J,SIEGEL RL,et al.Global cancer statistics 2020:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries［J］.CA:A Cancer Journal for Clinicians,2021,71(3):209-249.
［2］ BUSINELLO G,PARENTE P,MASTRACCI L,et al.The pathologic and molecular landscape of esophageal squamous cell carcinogenesis［J］.Cancers,2020,12(8):2160.
［3］ OCKLENBURG T,NEUMANN F,WOLF A,et al.In oxygen-deprived tumor cells ERp57 provides radioprotection and ensures proliferation via c-Myc,PLK1 and the AKT pathway［J］.Scientific Reports,2021,11(1):7199.
［4］ YANG Z,LIU J,SHI Q,et al.Expression of protein disulfide isomerase A3 precursor in colorectal cancer［J］.OncoTargets and Therapy,2018,11:4159-4166.
［5］ YOUNG HS,MCGOWAN LM,JEPSON KA,et al.Impairment of cell adhesion and migration by inhibition of protein disulphide isomerases in three breast cancer cell lines［J］.Bioscience Reports,2020,40(10):20193271.
［6］ LI S,ZHAO X,CHANG S,et al.ERp57 small interfering RNA silencing can enhance the sensitivity of drugresistant human ovarian cancer cells to paclitaxel［J］.International Journal of Oncology,2019,54(1):249-260.
［7］ HETTINGHOUSE A,LIU R,LIU CJ.Multifunctional molecule ERp57:From cancer to neurodegenerative diseases［J］.Pharmacology & Therapeutics,2018,181:34-48.
［8］ MOAVEN O,WANG T.Combined modality therapy for management of esophageal cancer:current approach based on experiences from east and west［J］.The Surgical Clinics of North America,2019,99(3):479-499.
［9］ 杜芳,侯晓东.XIAP基因对食管癌细胞黏附、侵袭和迁移能力的影响［J］.现代肿瘤医学,2020,28(17):2939-2942. DU F,HOU XD.Effects of XIAP gene on adhesion，invasion and migration of esophageal cancer cells［J］. Modern Oncology,2020,28(17):2939-2942.
［10］ LIU Y,WANG JX,NIE ZY,et al.Upregulation of ERp57 promotes clear cell renal cell carcinoma progression by initiating a STAT3/ILF3 feedback loop［J］.Journal of Experimental & Clinical Cancer Research,2019,38(1):439.
［11］ CHICHIARELLI S,ALTIERI F,PAGLIA G.ERp57/PDIA3:new insight［J］.Cellular & Molecular Biology Letters,2022,27(1):12.
［12］ SONG D,LIU H,WU J,et al.Insights into the role of ERp57 in cancer［J］.Journal of Cancer,2021,12(8):2456-2464.
［13］ LAM STT,LIM CJ.Cancer biology of the endoplasmic reticulum lectin chaperones calreticulin,calnexin and PDIA3/ERp57［J］.Progress in Molecular Biology and Translational Science,2021,59:181-196.
［14］ SHI W,HAN H,ZOU J,et al.Identification of dihydrotanshinone I as an ERp57 inhibitor with anti-breast cancer properties via the UPR pathway［J］.Biochemical Pharmacology,2021,190:114637.
［15］ POL JG,PLANTUREUX C,PEREZ-LANZON M,et al.PDIA3 as a potential bridge between immunogenic cell death and autoreactivity［J］.Oncoimmunology,2022,11(1):2130558.
［16］ WALCZAK A,GRADZIK K,KABZINSKI J,et al.The role of the ER-induced UPR pathway and the efficacy of its inhibitors and inducers in the inhibition of tumor progression［J］.Oxidative Medicine and Cellular Longevity,2019,2019:5729710.
［17］ YANG S,JACKSON C,KARAPETYAN E,et al.Roles of protein disulfide isomerase in breast cancer［J］.Cancers(Basel),2022,14(3):745.
［18］ POWELL LE,FOSTER PA.Protein disulphide isomerase inhibition as a potential cancer therapeutic strategy［J］.Cancer Medicine,2021,10(8):2812-2825.
［19］ ZHANG J,LI H,LI H,et al.Expression and prognostic significance of PDIA3 in cervical cancer［J］.International Journal of Genomics Maintains,2022,2022:4382645.
［20］ KONDO R,ISHINO K,WADA R,et al.Downregulation of protein disulfide-isomerase A3 expression inhibits cell proliferation and induces apoptosis through STAT3 signaling in hepatocellular carcinoma［J］.International Journal of Oncology,2019,54(4):1409-1421.
［21］ SAMANTA S,TAMURA S,DUBEAU L,et al.Expression of protein disulfide isomerase family members correlates with tumor progression and patient survival in ovarian cancer［J］.Oncotarget,2017,8(61):103543-103556.
［22］ SHIMODA T,WADA R,KURE S,et al.Expression of protein disulfide isomerase A3 and its clinicopathological association in gastric cancer［J］.Oncology Reports,2019,41(4):2265-2272.

Memo

Memo:
河南省医学科技攻关计划项目（编号：LHGJ20200851）
Last Update: 1900-01-01