|Table of Contents|

Peiminine induces apoptosis in breast cancer MCF-7 cells by regulating PI3K/Akt signaling pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 12
Page:
2180-2185
Research Field:
Publishing date:

Info

Title:
Peiminine induces apoptosis in breast cancer MCF-7 cells by regulating PI3K/Akt signaling pathway
Author(s):
YI Nian; WANG Li; WANG Song; BAO Zheng; HU Nie; LI Lihong; JIANG Zhongjun; XU Ge
Department of Thyroid and Breast Surgery,the Affiliated Nanhua Hospital,Hengyang Medical School,University of South China,Hunan Hengyang 421002,China.
Keywords:
peiminine; breast cancer; PI3K/Akt signaling pathway; apoptosis; mitochondrial
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2023.12.002
Abstract:
Objective:To investigate the effect of peiminine on apoptosis of breast cancer MCF-7 cells and its possible mechanism.Methods:MCF-7 cells were treated with different concentrations of peiminine or combined with PI3K inhibitor LY294002.MTT assay was used to detect the cell proliferation.Hoechst 33258 staining and Annexin V-FITC/PI flow cytometry assay were used to detect the cell apoptosis.JC-1 staining assay was used to detect mitochondrial membrane potential change.Western blotting was used to detect the protein expression of levels of PI3K(p110α),Akt,p-Akt(ser473),Bad,Bax,Bcl-2,cleaved-Caspase-3 in cells and Cyt C in mitochondrial and cytoplasm.Results:Peiminine inhibited MCF-7 cells proliferation in a time and concentration dependent manner.Meanwhile,peiminine induced morphological changes of apoptosis,promoted apoptosis,reduced mitochondrial membrane potential,and up-regulated the protein expression levels of Bad,Bax,cleaved-Caspase-3,and Cyt C in cytoplasm,down-regulated the protein expression levels of PI3K,p-Akt，Bcl-2,and Cyt C in mitochondrial.There was no significant effect on the expression level of Akt protein.However,peiminine combined with LY294002 intervention can enhance the promotion of MCF-7 cells apoptosis.Conclusion:Peiminine can promote the apoptosis of breast cancer MCF-7 cells,which may be achieved by blocking the mitochondrial apoptosis pathway mediated by PI3K/Akt signaling pathway.

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Memo

Memo:
湖南省自然科学基金（编号：2020JJ4552）；湖南省科技厅临床医疗技术创新引导计划（编号：2017SK50217）；湖南省衡阳市科技局指导性项目（编号：114）
Last Update: 1900-01-01