|Table of Contents|

Tandem mass tag-labeling proteomics combined with high-content screening technology reveal gastric cancer-related genes

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 12
Page:
2173-2180
Research Field:
Publishing date:

Info

Title:
Tandem mass tag-labeling proteomics combined with high-content screening technology reveal gastric cancer-related genes
Author(s):
LI Dehong1YANG Xingwen1ZHAO Fenghui2YAN Li1YANG Xiaoyan1YUAN Xiumei1WEI Lianhua1YANG Weilin3LU Yan1
1.Department of Clinical Laboratory;2.Department of Pathology,Gansu Provincial Hospital,Gansu Lanzhou 730000,China;3.Department of General Surgery,the First Hospital of Lanzhou University,Gansu Lanzhou 730000,China.
Keywords:
gastric cancerTMT-labeling quantitative proteomicshigh content screeninggastric cancer-related gene
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2023.12.001
Abstract:
Objective:To screen for the gastric cancer-related genes by tandem mass tag(TMT)-labeling quantitative proteomics analysis combined with high-content screening(HCS).Methods:Proteins differentially expressed between gastric cancer tissues and para-cancer tissues were screened by TMT-based quantitative proteomics analysis.Then the differentiated expressed proteins (DEPs) were used for enrichment analysis of GO functions and the KEGG pathways.The target genes were selected by consulting the PubMed database with the results of GO and KEGG.After lentivirus-mediated shRNA technology was employed to specifically knockdown the selected target genes in the gastric cancer cell line AGS,HCS was performed to evaluate cell proliferation and screen proliferation-related genes.mRNA levels,clonogenic assay and counting kit-8 (CCK8) assays were conducted to validated the genes.Results:A total of 1 008 DEPs(649 up-regulated proteins and 359 down-regulated proteins) were identified in gastric cancer and para-cancer tissues by TMT proteomics.The cell proliferation experiments by HCS showed SF3B4 (in 25 genes of interest) knockdown inhibited gastric cancer cell proliferation.Validations demonstrated that SF3B4 was a potential gene implicated in gastric cancer.Conclusion:TMT-labeling proteomics combined with HCS technology offers new possibilities for the screening of tumor-associated genes.This study suggests that SF3B4 was a potential gene related to gastric cancer cell proliferation,providing a new clue for the study of the pathogenesis of gastric cancer.

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Memo

Memo:
甘肃省自然科学基金项目(编号:20JR5RA146);甘肃省人民医院国家级科研项目培育计划(编号:19SYPYB-25);甘肃省卫生及计划生育委员会计划项目(编号:GSWSKY2017-15)
Last Update: 1900-01-01