|Table of Contents|

BSN-AS2 competitively binding miR-219a-5p to impact NSCLC proliferation,migration,invasion and apoptosis

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 11
Page:
2026-2031
Research Field:
Publishing date:

Info

Title:
BSN-AS2 competitively binding miR-219a-5p to impact NSCLC proliferation,migration,invasion and apoptosis
Author(s):
WANG Yun1; PENG Fei1; SONG Shan1; ZHANG Xiaoyun2; HU Wenxia1
1.Department of Respiratory Medicine；2.Department of Immune Rheumatology,Fourth Hospital of Hebei Medical University,Hebei Shijiazhuang 050011,China.
Keywords:
NSCLC; BSN-AS2; miR-219a-5p; NCI-H520
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2023.11.010
Abstract:
Objective:To investigate the mechanism of BSN-AS2 competitively binding miR-219a-5p regulating the proliferation,migration,invasion and apoptosis of NSCLC cells.Methods：Expression of BSN-AS2 and miR-219a-5p in NSCLC tissues and cell lines was detected by using quantitative real time polymerase chain reaction(qRT-PCR).The signal intensity of BSN-AS2 and miR-219a-5p was detected by using fluorescence in situ hybridization(FISH).Double-luciferase reporter gene assay was used to verify that miR-219a-5p targeted and regulated BSN-AS2.Transwell,CCK8 and Tunel were used to respectively detect effect of BSN-AS2-miR-219a-5p axis on cell invasion,migration,proliferation and apoptosis.The xenograft model of NSCLC in nude mice was constructed to verify the regulatory role of BSN-AS2-miR-219a-5p axis.Results:BSN-AS2 showed a high expression in NSCLC tissues and cell lines,whereas miR-219a-5p was low expressed,and BSN-AS2 competitively bound to miR-219a-5p.siBSN-AS2 inhibited the invasion,migration and proliferation of NCI-H520 cells and promoted apoptosis.The In-miR-219a-5p group promoted NCI-H520 cell invasion,migration and proliferation and inhibited cell apoptosis.siBSN-AS2+In-miR-219a-5p group promoted NCI-H520 cell invasion,migration and proliferation,and inhibited cell apoptosis compared with siBSN-AS2 group.BSN-AS2 enhanced tumor cell proliferation and tumor growth in vivo,while miR-219a-5p exerted inhibitory function.Conclusion:BSN-AS2 competitively binds to miR-219a-5p to facilitate NSCLC cell invasion,migration and proliferation,and inhibits apoptosis,while siBSN-AS2-miR-219a-5p axis can be a novel target of anti-NSCLC.

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河北省医学科学研究课题计划（编号：20221317）
Last Update: 2023-04-28