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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2023 11

Page:

2014-2020

Research Field:

Publishing date:

Info

Title:

The effect of circ_ 0000619 on the proliferation and glutamine metabolism of esophageal squamous cell carcinoma cells by regulating miR-595/GLS axis

Author(s):

ZHOU Yuxin¹; 2; WU Linken¹; 2; LI Sen¹; 2; CHEN Shihang¹; 2; QIAN Cuijuan¹; 2; YAO Jun¹; 2

1.Medical College,Taizhou University,Zhejiang Taizhou 318000,China;2.Institute of Tumor,Taizhou University,Zhejiang Taizhou 318000,China.

Keywords:

circ_0000619; esophageal squamous cell carcinoma; miR-595; GLS; glutamine metabolism

PACS:

R735.1

DOI:

10.3969/j.issn.1672-4992.2023.11.008

Abstract:

Objective:To investigate the effect and its possible mechanism of circ_0000619/miR-595/GLS axis on cell proliferation,migration,invasion and glutamine metabolism of human esophageal squamous cell carcinoma (ESCC) KYSE150 cells.Methods:qPCR was used to detect circ_0000619,miR-595 and glutaminase (GLS) mRNA expression levels in KYSE150 cells.Western blot was used to detect GLS protein levels in KYSE150 cells.Cell counting kit-8 (CCK-8)and Transwell assays were used to investigate KYSE150 cell proliferation,invasion and migration,respectively.Glutamine consumption,glutamate production and ATP production levels were detected by indicated assay kits.Bioinformatics analysis technology and dual luciferase reporter gene assay were used to analyze and detect the interaction among circ_0000619,miR-595 and GLS mRNA.Results:circ_0000619 was highly expressed in ESCC cell lines,and its parental gene DENND4A mRNA was highly expressed in esophageal cancer tissues (P<0.01).Knockdown of circ_0000619 significantly inhibited the cell proliferation,invasion and migration of KYSE150 cells (P<0.01),and significantly inhibited the glutamine consumption level,glutamate and ATP production level of KYSE150 cells (P<0.01).Knockdown of circ_0000619 significantly increased the expression of miR-595 (P<0.01) and inhibited the mRNA (P<0.01) and protein expression of GLS.Dual luciferase reporter gene assay demonstrated that circ_0000619 had targeted binding with miR-595,and miR-595 had targeted binding with GLS in KYSE150 cells(all P<0.01).Glutamine metabolism and cell proliferation,were significantly decreased by circ_0000619 knockdown in KYSE150 cells,and these effects were partly reversed by miR-595 inhibition or GLS over-expression.Conclusion:circ_ 0000619 can enhance glutamine metabolism and cell proliferation of ESCC cells through targeted regulation of miR-595/GLS axis,and may become a potential target for ESCC therapy.

References:

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Memo

Memo:

国家级大学生创新创业训练计划项目(编号：202210350057)；浙江省自然科学基金(编号：LTGY23H200001)；浙江省基础公益研究计划社会发展项目(编号：LGF22H160015)；浙江省大学生科技创新活动计划暨新苗人才计划项目（编号：2021R436018）；浙江省台州市科技计划项目(编号：21ywb74，22ywa59)

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Last Update: 2023-04-28