|Table of Contents|

Effects of regional lymphadenectomy on expression of CEA and VEGF-C in mice colorectal tumors

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 11
Page:
1992-1995
Research Field:
Publishing date:

Info

Title:
Effects of regional lymphadenectomy on expression of CEA and VEGF-C in mice colorectal tumors
Author(s):
WAN Shouwei1; 2; HUANG Guilin3; HAN Danqing3; HOU Jixue3
1.Medical College of Shihezi University,Xinjiang Shihezi 832000,China;2.Department of General Surgery,Shihezi People's Hospital,Xinjiang Shihezi 832000,China;3.The Second Department of General Surgery,First Affiliated Hospital of Medical College of Shihezi University,Xinjiang Shihezi 832000,China.
Keywords:
regional lymphadenectomy; CT26.WT; CEA; VEGF-C
PACS:
R735.3+4
DOI:
10.3969/j.issn.1672-4992.2023.11.004
Abstract:
Objective:To investigate the effects of regional lymphadenectomy on expression of CEA and VEGF-C in mice colorectal tumors.Methods:60 healthy BALB/c mice were divided into three groups:Virtual incision+PBS injection，15 mice(group A)，virtual incision+CT26.WT injection，30 mice(group B),and model group+CT26.WT injection，15 mice (group C).The model group mice were excised regional lymph nodes.The mice in group B were further divided into two groups:Group B1 (No regional lymphadenectomy was performed) and group B2(Regional lymphadenectomy was performed after 7 days of tumor implantation) with 15 mice in each group.The concentrations of CEA and VEGF-C in each group were detected by ELISA,and the tumor masses were weighed and measured.Results:The concentrations of CEA in group A,B1,B2 and C were (602.6±52.2) pg/mL,(640.3±37.6) pg/mL,(676.5±56.6) pg/mL and (698.9±45.7) pg/mL,VEGF-C concentrations in group A,B1,B2 and C were (153.5±13.0) pg/mL,(164.6±14.6) pg/mL,(177.1±15.4) pg/mL,(186.0±17.2) pg/mL.The concentrations of CEA and VEGF-C in group B and C were higher than those in group A（P<0.05）.Compared with group B1,the concentrations of CEA and VEGF-C in group B2 and group C were increased(P<0.05).The weight and volume of tumor mass were larger than those in group B1(P<0.05).Conclusion:Regional lymphadenectomy can up-regulate the expression of CEA and VEGF-C in mouse tumors,promote the growth and invasion of transplanted tumor.

References:

［1］BRAY F,FERLAY J,SOERJOMA I,et al.Global cancer statistics 2018：GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries［J］.CA Cancer J Clin,2018,68(6):394-424.
［2］KRUGER S,ILMER M,KOBOLD S,et al.Advances in cancer immunotherapy 2019-latest trends［J］.J Exp Clin Cancer Res,2019,38(1):268.
［3］WONG SL.Lymph node evaluation in colon cancer:assessing the link between quality indicators and quality［J］.Jama,2011,306(10):1139-1141.
［4］ZHUO CH,XU Y,YING MG,et al.FOXP3+ Tregs:heterogeneous phenotypes and conflicting impacts on survival outcomes in patients with colorectal cancer［J］.Immunol Res,2015,61(3):338-347.
［5］HE WZ,XIE QK,HU WM,et al.An increased number of negative lymph nodes is associated with a higher immune response and longer survival in colon cancer patients［J］.Cancer Management and Research,2018,10:1597-1604.
［6］QUAN Q,ZHU MS,LIU SS,et al.Positive impact of the negative lymph node count on the survival rate of stage III colon cancer with pN1 and right-side disease［J］.Journal of Cancer,2019,10(4):1052-1059.
［7］KUO YH,YOU JF,HUNG HY，et al.Number of negative lymph nodes with a positive impact on survival of stage III colon cancer; a retrospective observation study for right side and left side colon［J］.BMC Cancer,2022,22(1):126.
［8］NAKAMURA Y,FUJISAWA Y,OKIYAMA N,et al.Surgical damage to the lymphatic system promotes tumor growth via impaired adaptive immune response［J］.J Dermatol Sci,2018,90(1):46-51.
［9］BACZYNSKA D,WIETRZYK J,MADEJ J,et al.The tumorigenic potential of human CX-1 colon adenocarcinoma cells depends on carcinoembryonic antigen(CEACAM5) expression［J］.Cell Mol Biol Lett,2003,8(2):471-486.
［10］TIAN Y,WEN HX,WU HT,et al.The relationship between preoperative routine blood tests and postoperative tumor characteristics in patients with colorectal cancer［J］.Acta Academiae Medicinae Xuzhou,2021,41(2):113-118.
［11］敬秀平,刘珊,宋小平.结直肠癌患者的血清癌胚抗原、甲胎蛋白和糖类抗原19-9水平及临床意义［J］.癌症进展,2020,18(12):1244-1247. JING XP,LIU S,SONG XP.Expression of serum carcinoembryonic antigen,alpha-fetoprotein and carbohydrate antigen 19-9 in patients with colorectal cancer and its clinical significance［J］.Oncology Progress,2020,18(12):1244-1247.
［12］程煌荣，郑勇斌.结直肠癌患者肿瘤静脉血CTCs和CEA mRNA水平对异时性肝转移的预测价值［J］.现代肿瘤医学，2021,29（2）：263-266. CHENG HR,ZHENG YB.Detection of CTCs and CEA mRNA levels in tumor venous blood of patients with colorectal cancer for predicting metachronous liver metastasis［J］.Modern Oncology,2021,29（2）：263-266.
［13］MIAO H,RUAN S,SHEN M.VEGF-C in rectal cancer tissues promotes tumor invasion and metastasis［J］.J BUON,2018,23(1):42-47.
［14］TACCONI C,CORREALE C,GANDELLI A,et al.Vascular endothelial growth factor C disrupts the endothelial lymphatic barrier to promote colorectal cancer invasion［J］.Gastroenterology,2015,148(7):1438-1451.
［15］BI MM,SHANG B,WANG Z,et al.Expression of CXCR4 and VEGF-C is correlated with lymph node metastasis in non-small cell lung cancer［J］.Thoracic Cancer,2017,8(6):634-641.
［16］NAKAMURA H,ISHIGURO K,MORI T.Different immune functions of peripheral blood,regional lymph node,and tumor infiltrating lymphocytes in lung cancer patients［J］.Cancer,1988,62(12):2489-2497.
［17］CHEN YY,GE JY,MA D,et al.Immune-activated regional lymph nodes predict favorable survival in early-stage triple-negative breast cancer［J］.Front Oncol,2020,10:570981.
［18］VERONESI U,MARUBINI E,MARIANI L,et al.The dissection of internal mammary nodes does not improve the survival of breast cancer patients.30-year results of a randomised trial ［J］.Eur J Cancer,1999,35(9):1320-1325.
［19］VAN PUL KM,FRANSEN MF,VANDE VR,et al.Immunotherapy goes local:The central role of lymph nodes in driving tumor infiltration and efficacy［J］.Front Immunol,2021,12:643291.

Memo

Memo:
兵团科技计划项目（编号：2022ZD025）
Last Update: 2023-04-28