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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2023 11

Page:

1979-1985

Research Field:

Publishing date:

Info

Title:

m6A-modified circ_100367 mediates immune escape from papillary thyroid carcinoma by regulating the miR-885-5p axis

Author(s):

FENG Haigang¹; LIU Guowen²; CAO Hong¹

1.Department of Thyroid and Breast Surgery,the Second Affiliated Hospital,Hengyang Medical School,University of South China,Hunan Hengyang 421001,China;2.Department of Thyroid and Breast Surgery,Shenzhen Second People's Hospital,Guangdong Shenzhen 518025,China.

Keywords:

circ_100367; m6A modification; miR-885-5p; papillary thyroid carcinoma; immune escape

PACS:

R736.1

DOI:

10.3969/j.issn.1672-4992.2023.11.002

Abstract:

Objective:To investigate the effect of m6A-modified circ_100367 on immune escape of papillary thyroid carcinoma(PTC) and its possible mechanism.Methods:Expression levels of circ_100367 and miR-885-5p in PTC patients' cancer tissues and their cancer cell lines were detected by qRT-PCR assay.si-circ_100367 interference plasmid (si-circ_100367) and its negative control interference plasmid (si-NC),circ_100367 overexpression plasmid (oe-circ_100367) and its negative control plasmid (oe-NC),and miR-885-5p mimics and negative control NC mimics were transfected into TPC1 cells and divided into Blank group,si-circ_100367 group,si-NC group,oe-circ_100367 group,oe-NC group,miR-885-5p mimics group and NC mimics group.The expression levels of circ_100367 and miR-885-5p in TPC1 cells of each group was detected by qRT-PCR assay.The dual-luciferase reporter system was used to verify the targeted regulation of miR-885-5p by circ_100367.The m6A methylation level of circ_100367 was detected by MeRIP-qPCR.The invasive ability of cells was detected by Transwell method.The cell migration ability was detected by wounding healing assay.The expression levels of FASL,IDO1,PD-L2 and PD-L1 were detected by Western blot.Results:The expression level of circ_100367 was significantly increased in the cancer tissures of PTC patients and its cancer cell lines K1,TPC1 and IHH4 (P<0.05),while the expression level of miR-885-5p was significantly decreased (P<0.05).The results of the dual-luciferase reporter system showed that circ_100367 targeted and negatively regulated miR-885-5p.The results of MeRIP assay showed that circ_100367 was rich in m6A methylation,and compared with NC mimics,the m6A methylation level of circ_100367 in miR-885-5p mimics cells was significantly lower (P<0.05).Compared with Blank or si-NC,si-circ_100367 cell invasion number,cell wound healing rate and FASL,PD-L1,PD-L2,IDO1 protein expression levels were significantly decreased (P<0.05).Compared with Blank or oe-NC,oe-circ_100367 cell invasion number,cell wound healing rate and protein expression levels of FASL,PD-L1,PD-L2,IDO1 were significantly increased (P<0.05).Compared with Blank or NC mimics,the invasion number,cell wound healing rate of miR-885-5p mimics and expression levels of FASL,PD-L1,PD-L2,IDO1 proteins were significantly decreased (P<0.05).Conclusion:The m6A methylated circ_100367 can induce PTC cell invasion,affect the expression of PD-L1 and promote immune escape,which may be related to the competitive binding of m6A methylated circ_100367 and miR-885-5p.

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