|Table of Contents|

Expression of zinc finger protein 883 in gastric cancer tissues and biological function

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 10
Page:
1854-1858
Research Field:
Publishing date:

Info

Title:
Expression of zinc finger protein 883 in gastric cancer tissues and biological function
Author(s):
ZHANG Ting1; LIU Chang2; YU Junhui2; ZHAO Wei2
1.Department of Outpatient;2.Department of General Surgery,the First Affiliated Hospital of Xi'an Jiaotong University,Shaanxi Xi'an 710061,China.
Keywords:
gastric cancer; zinc finger protein 883; poor prognosis; oncogene
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2023.10.015
Abstract:
Objective:To explore the expression of zinc finger protein 883 (ZNF883) in gastric cancer tissues and its prognostic significance,and observe its effects on the proliferation,migration and invasion of gastric cancer cells.Methods:Based on TCGA data,the GEPIA network platform was used to analyze the expression difference of ZNF883 mRNA between gastric cancer tissues and normal gastric tissues and its correlation with patient survival.The expression level of ZNF883 protein in gastric cancer tisues and corresponding tumor-adjacent tissues was detected by Western blotting (WB).ZNF883 shRNA was transfected into human gastric cancer cells AGS and NCI-N87 and the knockdown efficiency was detected by WB.CCK-8 and Transwell assays were used to detect cell proliferation,migration and invasion,and the protein expression of cyclin D1 (CCND1),cyclin dependent kinase 4 (CDK4) and matrix metallopeptidase 2/9 (MMP2/9) was detected by WB.Results:TCGA data analysis found that the expression of ZNF883 mRNA in gastric cancer tissues was significantly higher than that in normal gastric tissues.ZNF883 protein level was also upregulated in gastric cancer tissues.Survival analysis demonstrated that gastric cancer patients with high ZNF883 mRNA expression had an obvious shorter disease-free survival and overall survival.ZNF883 knockdown prominently inhibited the proliferation,migration and invasion of AGS and NCI-N87 cells.In addition,ZNF883 knockdown markedly reduced the protein levels of CCND1,CDK4 and MMP2/9 in gastric cancer cells.Conclusion:ZNF883 is a novel gastric cancer driver gene.The high expression of ZNF883 in gastric cancer tissues indicates a poor prognosis for patients.ZNF883 may enhance the proliferation,migration and invasion of gastric cancer cells by promoting the expression of CCND1,CDK4 and MMP2/9.

References:

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Memo

Memo:
National Natural Science Foundation of China(No.82002547）；国家自然科学基金资助项目（编号：82002547）
Last Update: 1900-01-01