|Table of Contents|

Inhibition effect of miR-204 on proliferation,migration and invasion of acute lymphoblastic leukemia CEM/C1 cells by targeting SOX4

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 10
Page:
1826-1832
Research Field:
Publishing date:

Info

Title:
Inhibition effect of miR-204 on proliferation,migration and invasion of acute lymphoblastic leukemia CEM/C1 cells by targeting SOX4
Author(s):
YAN Yulan1; FU Tingting2; HAN Lei3; HAN Feng1
1.Department of Clinical Laboratorial Examination,the First Affiliated Hospital of Hainan Medical University,Hainan Haikou 570102,China;2.School of Tropics and Laboratory,Hainan Medical University,Hainan Haikou 571199,China;3.Laboratory Department,Haikou Maternal and Child Health Hospital,Hainan Haikou 570102,China.
Keywords:
miR-204; SOX4; acute lymphoblastic leukemia; migration; invasion
PACS:
R733.71
DOI:
10.3969/j.issn.1672-4992.2023.10.010
Abstract:
Objective:To investigate the effect of miR-204 on the proliferation,migration and invasion of acute lymphoblastic leukemia (ALL) CEM/C1 cells and its targeting relationship with SOX4.Methods:ALL cell lines CEM/C1 was selected.According to the different transfection substances,they were divided into NC group (CEM/C1 cells),miR-NC group (miR-NC),miR-204 group (miR-204-mimic),si-NC group (transfected with si-NC),si-SOX4 group (transfected with si-SOX4),miR-204+pcDNA group (transfected with miR-204-mimic+pcDNA) and miR-204+pcDNA-SOX4 group (transfected with miR-204-mimic+pcDNA-SOX4).Real time fluorescent quantitative PCR was used to detect the expression of miR-204.MTT was used to detect cell proliferation.Transwell chamber test was used to detect cell migration and invasion.Western blot was used to detect the expression level of SOX4.Luciferase assay was used to verify the relationship between miR-204 and SOX4.Results:At 48 h and 72 h,the cell viability,migration and invasion of miR-204 group were significantly lower than those of NC group and miR-NC group (P＜0.05),apoptosis rate was higher than those of NC group and miR-NC group (P＜0.05).At 48 h and 72 h,the cell viability,migration and invasion in si-SOX4 group were significantly lower than those in NC group and si-NC group (P＜0.05),apoptosis rate was higher than those of NC group and si-NC group (P＜0.05).The cell viability,migration and invasion of miR-204+pcDNA-SOX4 group were higher than those of miR-204+pcDNA group and miR-204 group at 48 h and 72 h (P＜0.05),apoptosis rate was lower than those of miR-204+pcDNA group and miR-204 group (P＜0.05).The luciferase reporter assay showed that miR-204 could reduce the activity of luciferase in SOX4 wild-type vectors (P＜0.05).But there was no statistically significant difference in SOX4 mutant vectors (P>0.05).Conclusion:miR-204 can negatively regulate SOX4 and inhibit the proliferation,migration and invasion of ALL CEM/C1 cells.

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