|Table of Contents|

Abnormal expression of cystathionine β synthase in prostate cancer and its regulation on cells proliferation

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 10
Page:
1811-1815
Research Field:
Publishing date:

Info

Title:
Abnormal expression of cystathionine β synthase in prostate cancer and its regulation on cells proliferation
Author(s):
YANG Shiwei; LAI Weiqi; SONG Caiyong; CHENG Tianmu; HU Yu; TANG Qingfeng
Department of Urology,Xindu District People's Hospital of Chengdu City,Sichuan Chengdu 610500,China.
Keywords:
cystathionine β synthase; prostate cancer; cell proliferation; tumor growth
PACS:
R737.25
DOI:
10.3969/j.issn.1672-4992.2023.10.007
Abstract:
Objective:To investigate the expression pattern of cystathionine β synthase (CBS) in prostate cancer cells,and determine the impact of CBS on the proliferation of prostate cancer cells as well as the related mechanism.Methods:The CBS mRNA expression of 492 prostate cancer tissues and 152 normal tissues obtained from TCGA and GTEx databases was analyzed by the GEPIA tool.The CBS mRNA expression of 497 prostate cancer tissues and 52 normal tissues obtained from TCGA was analyzed by the ULCAN tool.Prostate cancer cell lines DU145,PC3,LNCaP,and C4-2 were cultured in RPIM-1640 medium,and Western blot was used to detect the expression of CBS protein.DU145 cells were transfected with Control shRNA,CBS shRNA#1 or #2,respectively.The proliferation of DU145 cells was determined by colony formation assay and BrdU labeling assay.Western blot was used to measure the protein expression of AKT,mTOR and S6K,and the phosphorylation levels of AKT S473,mTOR S2448 and S6K T421/S424.DU145 cells stably expressing Control shRNA and CBS shRNA#1 or #2 were subcutaneously injected into mice to establish a prostate cancer xenograft model and the tumor growth was observed.Results:Analysis of the online protein expression database revealed that the expression of CBS mRNA in prostate cancer tissues was significantly increased compared to normal prostate tissues.CBS protein was expressed in all measured prostate cancer cell lines with the highest in DU145 cells.Compared with Control shRNA group,the average number of clones formed in CBS shRNA#1 or #2 groups was significantly decreased by colony formation assay (23±2.309 vs 5.667±1.453,6.333±1.856),and the intensity of BrdU labeling signal in CBS shRNA#1 or #2 groups was significantly decreased by BrdU labeling assay (211.3±25.31 vs 90±10.6,113±15.39).Mouse xenograft model showed that treatment with CBS shRNA#1 or #2 significantly reduced the growth rate of xenografts.On day 27,the average volume of Control shRNA,CBS shRNA#1 or #2 groups was (840.4±37.48)mm3 vs (415.9±34.88)mm3,(297.6±25.74)mm3,respectively.Compared with Control shRNA group,there was no obvious change in the expression level of AKT,mTOR,S6K proteins in CBS shRNA#1 or #2 groups,but the phosphorylation level of AKT S473,mTOR S2448 and S6K T421/S424 were significantly decreased.Conclusion:CBS expression was upregulated in prostate cancer.Knockdown of CBS inhibited the proliferation of prostate cancer cells,and inhibited the phosphorylation of AKT,mTOR and S6K.

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