|Table of Contents|

Lycorine regulates autophagy and inhibits the proliferation of esophageal cancer cells through TGF-β/Smad signal pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 10
Page:
1799-1804
Research Field:
Publishing date:

Info

Title:
Lycorine regulates autophagy and inhibits the proliferation of esophageal cancer cells through TGF-β/Smad signal pathway
Author(s):
MA Yongchao1; 2; REN Liping2; 3; JIN Shaoju2; 3; XU Songtao2; 4
1.Academic Affairs Office;3.Department of Pharmacy;4.Medical Department,Luohe Medical College,Henan Luohe 462002,China;2.Henan Province Engineering ＆ Technology Research Center of Foods for Special Medical Purpose,Henan Luohe 462002,China.
Keywords:
lycorine; TGF-β/Smad signal pathway; esophageal cancer; autophagy
PACS:
R735.1
DOI:
10.3969/j.issn.1672-4992.2023.10.005
Abstract:
Objective:To investigate the effect of lycorine on the proliferation of esophageal cancer cells and analyze its mechanism.Methods:The inhibition rate of cell proliferation was analyzed by cell counting kit-8 (CCK-8).Autophagy vesicles were detected by MDC staining.The levels of LC3II/LC3I,Beclin-1,transforming growth factor-β (TGF-β) and p-Smad2/Smad2 were analyzed by Western blot.The effect of 3-MA on autophagy and apoptosis regulated by lycorine was observed.The tumorigenesis experiment of nude mice verified the effect of allicin on tumor weight,tumor volume,autophagy and p-Smad2/Smad2 level in nude mice.Results:Lycorine at 2、4、8、16、32、64 μmol/L inhibited the proliferation of ECA109 cells (F= 22.412,P＜0.05),and the IC50 value was (15.56±2.16)μmol/L.4,8 and 16 μmol/L lycorine promoted the production of autophagy vesicles,increased the levels of LC3II/LC3I and Beclin-1,and decreased the levels of TGF-β1 and p-Smad2/Smad2.3-MA could reverse the effects of lycorine on autophagy,apoptpsis,TGF-β/Smad signal pathway and Bcl-2/Bax signal pathway.100 mg/kg lycorine could reduce tumor volume,tumor mass,down-regulate tumor p-Smad2/Smad2 level and up-regulate LC3II/LC3I level.3-MA could reverse the effect of lycorine on tumor volume,tumor mass and the level of LC3II/LC3I and p-Smad2/Smad2.Conclusion:Lycorine can inhibit the proliferation of ECA109 cells,promotes apoptosis and has obvious anti-tumor effect on ECA109 fine cell transplanted tumor in vivo.The mechanism is related to the activation of autophagy by regulating TGF-β/Smad signal pathway.

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河南省高等学校重点科研项目（编号：22B310006）;漯河医学高等专科学校创新创业发展能力提升工程（编号：2021LYZKJXM024）;漯河医学高等专科学校2020年度创新创业发展能力提升工程项目（编号：2020-LYZKYYB005）
Last Update: 1900-01-01