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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2023 10

Page:

1785-1793

Research Field:

Publishing date:

Info

Title:

LncRNA LINC00958 promotes the proliferation,invasion and migration of papillary thyroid carcinoma cells by targeting miR-490-3p

Author(s):

YUAN Ning¹; WANG Xiaojuan¹; BAI Yajun²; HE Minghai¹; WANG Feng¹

1.Department of Endocrinology;2.Department of Sensory Control,Nanchong Central Hospital,Sichuan Nanchong 637003,China.

Keywords:

LncRNA LINC00958; miR-490-3p; papillary thyroid cancer; migration; invasion

PACS:

R736.1

DOI:

10.3969/j.issn.1672-4992.2023.10.003

Abstract:

Objective:To study the effect and its mechanism of lncRNA LINC00958 on thyroid cancer cells.Methods:TPC-1 and K-1 cells were divided into Control,shRNA-NC,shRNA-LINC00958-1,shRNA-NC+inhibitor-NC,shRNA-LINC00958-1+inhibitor-NC and shRNA-LINC00958-1+miR-490-3p inhibitor,respectively.Tumor-bearing mice with implantation of TPC-1 or K-1 cells were divided into shRNA-NC,shRNA-LINC00958-1,shRNA-NC+inhibitor-NC,shRNA-LINC00958-1+inhibitor-NC and shRNA-LINC00958-1+miR-490-3p inhibitor.The expression levels of LINC00958 and miR-490-3p were detected by real-time fluorescence quantitative PCR (RT-qPCR).Cell viability was detected by CCK-8 assay and Western blot was used to detect protein expression level.The migration and invasion of TPC-1 and K-1 cells were detected by wound healing and Transwell assay,respectively.Cell proliferation was detected by clone formation assay.The targeting relationship between LINC00958 and miR-490-3p was detected by dual luciferase reporting assay.The mass and volume of tumor were measured.Results:The results showed that compared with normal tissues,LINC00958 was highly expressed in thyroid cancer tissues.miR-490-3p showed low expression in TPC-1 and K-1 cells compared with Nthy-ori 3-1 cells.After silencing LINC00958,the proliferation,invasion and migration of papillary thyroid cancer cells were significantly decreased.miR-490-3p silencing blunted the effect of LINC00958 silencing on these malignant behaviors in vitro and suppressing tumor growth.Conclusion:LINC00958 silencing can inhibit proliferation,migration and invasion of thyroid cancer cells,as well as tumor growth by targeting miR-490-3p.

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