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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2023 09

Page:

1653-1659

Research Field:

Publishing date:

Info

Title:

Establishment of a prognosis model of laryngeal carcinoma with Golgi apparatus-related genes

Author(s):

KANG Wei¹; WU Qun²; KOU Bo¹; ZHANG Shaoqiang¹

1.Department of Otolaryngology Head and Neck Surgery,the First Affiliated Hospital of Xi'an Jiaotong University,Shaanxi Xi'an 710061,China;2.Department of Ophthalmology and Otorhinolaryngology,Yangxian Hospital of Traditional Chinese Medicine,Shaanxi Hanzhong 723300,China.

Keywords:

Golgi apparatus; laryngeal cancer; TCGA database

PACS:

R739.65

DOI:

10.3969/j.issn.1672-4992.2023.09.013

Abstract:

Objective:To screen Golgi apparatus-related prognosis genes based on TCGA database and further construct a risk model for the prognosis of laryngeal cancer.Methods:Golgi apparatus-related gene maps were obtained from the MSigDB database.The expression profiles of Golgi apparatus-related genes of laryngeal cancer and adjacent tissues in TCGA database were screened,and differential expressed genes between cancer and adjacent tissues were analyzed by limma method.Golgi apparatus-related gene expression profile combined with clinical data were used for single factor independent prognostic analysis to obtain genes related to prognosis.The cross analysis of differential genes and prognostic genes was carried out,and the obtained genes were then used to construct the model by LASSO regression.Kaplan-Meier method was used to analyze the prognosis of high-risk group and low-risk group.ROC curve verified the reliability of the prediction model.qRT-PCR and Western blot were used to identify the difference of gene expression between laryngeal carcinoma and adjacent tissues.Results:1 659 Golgi apparatus-related genes were obtained from the MSigDB database,and 1 084 Golgi apparatus-related gene expression matrices were obtained by combining with laryngeal cancer data in the TCGA database.77 upregulated and 190 downregulated Golgi apparatus-related genes were screened through differential analysis.By single factor prognostic analysis of 1 084 Golgi related genes,8 prognostic inhibitors and 15 prognostic promoters of laryngeal carcinoma were acquired.Through cross analysis,we finally got 1 highly expressed prognosis inhibitor in adjacent tissues of laryngeal carcinoma and 5 highly expressed prognosis promoting factors in laryngeal carcinoma tissues.These 6 genes were used for LASSO regression analysis to build a model.Kaplan-Meier analysis showed that the prognosis of high-risk group was worse than that of low-risk group (P£¼0.05).ROC curve verified that the reliability of the prediction model was good.qRT-PCR and Western blot confirmed that the expression of TMC8 in laryngeal carcinoma was lower than that in adjacent tissues,and the expression of SLC35C1,PDGFA,GALNT2 and ITGA5 in laryngeal carcinoma was higher than that in adjacent tissues.Conclusion:By using bioinformatics analysis method and experimental validation,a prediction model for the prognosis of laryngeal cancer was screened and constructed,which provides new ideas and methods for the research,diagnosis and treatment of this disease.

References:

£Û1£Ý JOHNSON DE,BURTNESS B,LEEMANS CR,et al.Head and neck squamous cell carcinoma£ÛJ£Ý.Nat Rev Dis Primers,2020,6(1):92.
£Û2£Ý COHEN EEW,BELL RB,BIFULCO CB,et al.The society for immunotherapy of cancer consensus statement on immunotherapy for the treatment of squamous cell carcinoma of the head and neck (HNSCC) £ÛJ£Ý.J Immunother Cancer,2019,7(1):184.
£Û3£Ý ECHANIQUE KA,EVANS LK,HAN AY,et al.Cancer of the larynx and hypopharynx£ÛJ£Ý.Hematol Oncol Clin North Am,2021,35(5):933-947.
£Û4£Ý COSTA SNL,FERNANDES FCGM,SOUZA DLB,et al.Incidence and mortality by larynx cancer in central and south America£ÛJ£Ý.Rev Gaucha Enferm,2021,42:e20190469.
£Û5£Ý DENG Y,WANG M,ZHOU L,et al.Global burden of larynx cancer,1990-2017:estimates from the global burden of disease 2017 study£ÛJ£Ý.Aging (Albany NY),2020,12(3):2545-2583.
£Û6£Ý LIU J,HUANG Y,LI T,et al.The role of the Golgi apparatus in disease (review) £ÛJ£Ý.Int J Mol Med,2021,47(4):38.
£Û7£Ý LI H,ZHANG P,LUO J,et al.Chondroitin sulfate-linked prodrug nanoparticles target the golgi apparatus for cancer metastasis treatment£ÛJ£Ý.ACS Nano,2019,13(8):9386-9396.
£Û8£Ý TAO Y,YANG Y,ZHOU R,et al.Golgi apparatus:an emerging platform for innate immunity£ÛJ£Ý.Trends Cell Biol,2020,30(6):467-477.
£Û9£Ý ZHANG M,XU N,XU W,et al.Potential therapies and diagnosis based on golgi-targeted nano drug delivery systems£ÛJ£Ý.Pharmacol Res,2022,175:105861.
£Û10£Ý GARC¦PA-LE¦VN FJ,GARC¦PA-ESTEPA R,ROMERO-TABARES A,et al.Treatment of advanced laryngeal cancer and quality of life.Systematic review£ÛJ£Ý.Acta Otorrinolaringol Esp (Engl Ed),2017,68(4):212-219.
£Û11£Ý LUJAN P,CAMPELO F.Should I stay or should I go? Golgi membrane spatial organization for protein sorting and retention£ÛJ£Ý.Arch Biochem Biophys,2021,707:108921.
£Û12£Ý ZHANG X.Alterations of golgi structural proteins and glycosylation defects in cancer£ÛJ£Ý.Front Cell Dev Biol,2021,9:665289.
£Û13£Ý MARTINS ¦BM,RAMOS CC,FREITAS D,et al.Glycosylation of cancer extracellular vesicles:capture strategies,functional roles and potential clinical applications£ÛJ£Ý.Cells,2021,10(1):109.
£Û14£Ý JOHNSTON N,WELLS CW,SAMUELS TL,et al.Rationale for targeting pepsin in the treatment of reflux disease£ÛJ£Ý.Ann Otol Rhinol Laryngol,2010,119(8):547-558.
£Û15£Ý Ф¹úá¶,Ö£Òå²Ê,ÎâÇ飬µÈ.¸ß¶û»ùÌåÕûºÏĤµ°°×4ÔÚÈËÍ·¾±°©Öеıí´ï¼°ÁÙ´²ÒâÒå£ÛJ£Ý.ÏÖ´ú¿ÚǻҽѧÔÓÖ¾£¬2020,34(05):277-280. XIAO GX,ZHENG YC,WU Q,et al.Expression and clinical significance of golgi integral membrane protein 4 in head and neck cancer£ÛJ£Ý.Journal of Modern Stomatology,2020,34(05):277-280.
£Û16£Ý LIN B,WANG S,YAO Y,et al.Comprehensive co-expression analysis reveals TMC8 as a prognostic immune-associated gene in head and neck squamous cancer£ÛJ£Ý.Oncol Lett,2021,22(1):498.
£Û17£Ý YU Y,WANG Z,ZHENG Q,et al.GALNT2/14 overexpression correlate with prognosis and methylation:potential therapeutic targets for lung adenocarcinoma£ÛJ£Ý.Gene,2021,790:145689.
£Û18£Ý ÌÆÓñÁ«,³ÂÆôÍþ,Τ¹ó£¬µÈ.PDGFAÔÚ±ÇÑÊ°©ÖеIJîÒì±í´ï¼°Æ书ÄÜ·ÖÎö£ÛJ£Ý.ÓÒ½­Ãñ×åҽѧԺѧ±¨£¬2021,43(01):46-50. TANG YL,CHEN QW,WEI G,et al.Differential expression and functional analysis of PDGFA in naspharyngeal carcinoma£ÛJ£Ý.Journal of Youjiang Medical University for Nationalities,2021,43(01):46-50.

Memo

Memo:

National Natural Science Foundation of China(No.81802934£©£»¹ú¼Ò×ÔÈ»¿Æѧ»ù½ðÇàÄê¿Æѧ»ù½ðÏîÄ¿£¨±àºÅ£º81802934£©

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Last Update: 2023-03-30