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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2023 09

Page:

1639-1646

Research Field:

Publishing date:

Info

Title:

Cisatracurium regulates proliferation,migration and invasion of gastric cancer AGS cells via miR-526b-3p/FMNL3 pathway

Author(s):

LI Zhenzhen¹; CHEN Feiren¹; WU Hongfa¹; LIN Hui²

1.Haikou Hospital of the Maternal and Child Health,Hainan Haikou 570102,China;2.Department of Anesthesiology,Hainan Provincial People's Hospital,Hainan Haikou 570311,China.

Keywords:

cisatracurium; miR-526b-3p; FMNL3; gastric cancer; proliferation; migration; invasion

PACS:

R735.2

DOI:

10.3969/j.issn.1672-4992.2023.09.011

Abstract:

Objective：To investigate the effect and mechanism of cisatracurium on the proliferation,migration and invasion of gastric cancer AGS cells.Methods:A total of 30 cases of gastric cancer tissues and corresponding normal gastric mucosal tissues from January 2019 to January 2022 were selected.The experiment was divided into the control group (normal cultured cells),cisatracurium low (10 mmol/L),middle (20 mmol/L),and high (30 mmol/L) concentration group,miR-NC group,miR-526b-3p group,anti-miR-NC group,anti-miR-526b-3p group,Cis+anti-miR-NC group,Cis+anti-miR-526b-3p group.The cells in Cis+anti-miR-NC group,Cis+anti-miR-526b-3p group were transfected with miR-526b-3p negative control plasmid,miR-526b-3p inhibitor and then treated with 30 mmol/L cisatracurium.Cell proliferation inhibition rate was detected by MTT assay.Transwell assay was used to detect cell migration and invasion.Real-time quantitative PCR (RT-qPCR) was used to detect miR-526b-3p and FMNL3 mRNA expression.Luciferase reporter assay was used to detect the targeting relationship of miR-526b-3p and FMNL3.Results:The expression level of miR-526b-3p in gastric cancer tissues was lower than that in normal tissues,and the expression level of FMNL3 protein and mRNA was higher than that in normal tissues(P＜0.05).Compared with control group,the cell proliferation inhibition rate in cell treated with cisatracurium low,middle and high concentrations group was increased,the number of migration and invasion cells was decreased,the expression of miR-526b-3p was increased,and the expression of FMNL3 mRNA and protein was significantly decreased (P＜0.05).Overexpression of miR-526b-3p inhibits the proliferation,migration and invasion of AGS cells.miR-526b-3p targeted the regulation of FMNL3 expression,and inhibition of miR-526b-3p expression reversed the inhibitory effect of cisatracurium on AGS cells.Conclusion:Cisatracurium can inhibit the proliferation,migration and invasion of gastric cancer AGS cells,and its mechanism may be related to miR-526b-3p and FMNL3.

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Memo

Memo:

海南省自然科学基金项目(编号：821RC1112)

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Last Update: 2023-03-30