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The effect of aloe-emodin nanoliposomes combined with 5-FU on the proliferation,apoptosis,invasion and migration of human tongue squamous cell carcinoma CAL-27 cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 09
Page:
1626-1632
Research Field:
Publishing date:

Info

Title:
The effect of aloe-emodin nanoliposomes combined with 5-FU on the proliferation,apoptosis,invasion and migration of human tongue squamous cell carcinoma CAL-27 cells
Author(s):
DU Wenjuan; WANG Xiaoci; ZHOU Xiaohong; LI Yihang; YUAN Tianshuo; ZHAO Yanmei; MENG Peisong
Department of Stomatology,the Fourth Affiliated Hospital of Harbin Medical University,Heilongjiang Harbin 150001,China.
Keywords:
aloe-emodin nanoliposomes; 5-fluorouracil; oral cancer; cell apoptosis
PACS:
R739.8
DOI:
10.3969/j.issn.1672-4992.2023.09.009
Abstract:
Objective：To investigate the effect of aloe-emodin nanoliposome(AE-L) combined with 5-fluorouracil(5-FU) on human tongue squamous cell carcinoma CAL-27 cells.Methods:AE-L was prepared by reverse-phase evaporation.CCK-8 was used to detect the effect of different concentrations of AE-L and 5-FU on the inhibition rate of CAL-27 cells.Clone formation assay was used to detect cell proliferation.Scratch repair assay was used to detect cell migration ability.Cell invasion ability was detected by Transwell invasion assay.Hoechst 33342 was used to detect cell apoptosis.The expression of Bcl-2 and Caspase-3 mRNA was detected by Real-time fluorescent quantitative PCR(qRT-PCR ).Results:AE-L combined with 5-FU can significantly inhibit the proliferation of CAL-27 cells,reduce cell migration ability,and also reduce cell invasion ability.Hoechst 33342 staining showed that the number of apoptotic cells in combination group was significantly higher than that in blank control group and single drug group.The expression of anti-apoptotic gene Bcl-2 was significantly decreased and the expression of apoptotic gene Caspase-3 was significantly increased by qRT-PCR in combination group.Conclusion:AE-L combined with 5-FU inhibit human tongue squamous cell carcinoma CAL-27 cells,and its mechanism may be associated with apoptosis.

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Last Update: 2023-03-30