|Table of Contents|

Expression of long non-coding RNA FGD5-AS1 in lung cancer tissue and its effect on cell proliferation

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 09
Page:
1619-1625
Research Field:
Publishing date:

Info

Title:
Expression of long non-coding RNA FGD5-AS1 in lung cancer tissue and its effect on cell proliferation
Author(s):
WANG Jing1; YANG Lijie2; SONG Zheng2; ZHANG Yulu3; WANG Ruiyu3; LI Jun1
1.Department of Clinical Medicine,Dali University,Yunnan Dali 671000,China;2.Cardio-Thoracic Surgery,the First Affiliated Hospital of Dali University,Yunnan Dali 671000,China；3.Department of Public Health,Dali University,Yunnan Dali 671000,China.
Keywords:
lung cancer; long non-coding RNA; cell proliferation; miRNA
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2023.09.008
Abstract:
Objective：To research the expression of long non-coding RNA(lncRNA) FGD5-AS1 in lung cancer and its effect on cell proliferation of lung cancer.Methods：RNA-seq was used to analyze the expression changes of lncRNA and microRNA(miRNA) in lung cancer tissues,and the candidate lncRNA FGD5-AS1 was determined according to the expression changes.RT-qPCR was used to detect and analyze the expression of candidate FGD5-AS1 in lung cancer tissues and lung cancer cells.Immunofluorescence assay was used to analyze the expression of Ki-67 in lung cancer tissues.The correlation between FGD5-AS1 and Ki-67 expression in lung cancer tissues was analyzed by linear regression.Bioinformatics prediction was combined with miRNA sequencing and luciferase detection was used to analyze the binding possibility of FGD5-AS1 and miR-129-5p.A549 cells were divided into blank control group(Control),si-FGD5-AS1 group,miR-mimics group(miR-129-5p mimics),si-FGD5 -AS1 and miR-mimics combined group.si-FGD5-AS1 and miR-129-5p mimics were respectively or jointly transfected into A549 cells by Lipofectamine 3000.24 h later,Cell viability was detected by CCK-8,and apoptosis was detected by Annexin V-FITC/PI double staining,and the expression of apoptosis related proteins(Bad,Bcl-2,Cleaved caspase-3) was detected by Western blot.Results：The expression of FGD5-AS1 in lung cancer tissues and lung cancer cells was significantly increased(P＜0.01),and there was a positive correlation between FGD5-AS1 and Ki-67 expression(r=0.641 8,P=0.000).The expression of miR-129-5p in lung cancer tissues and cells was significantly decreased(P＜0.01).Bioinformatics prediction and luciferase detection confirmed that there was a binding site between FGD5-AS1 and miR-129-5p.In lung cancer tissues,the expression of miR-129-5p was negatively correlated with that of Ki-67(r=-0.752 8,P=0.000).Compared with the Control group,si-FGD5-AS1 significantly inhibited the proliferation of A549 cells(P＜0.01) and promoted apoptosis(P＜0.01),and significantly increased the expression of Bad and Cleaved caspase-3(all P＜0.01),and significantly decreased the expression of Bcl-2(P＜0.05).Compared with si-FGD5-AS1 group,si-FGD5-AS1 and miR-mimics combined group could further inhibit cell proliferation(P＜0.01),promote cell apoptosis(P＜0.01).The expression of Bad and Cleaved caspase-3 was significantly increased(all P＜0.01).The expression of Bcl-2 was significantly decreased(P＜0.01).Conclusion：The expression of FGD5-AS1 is significantly up-regulated in lung cancer,which may promote the proliferation of lung cancer cells by inhibiting the expression of hsa-miR-129-5p and participate in the occurrence and progression of lung cancer.

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Memo

Memo:
大理大学博士科研启动基金项目（编号:KYBS2018018）
Last Update: 2023-03-30