|Table of Contents|

Long non-coding RNA LINC00313 promotes proliferation and migration of lung cancer cells through ferroptosis resistance

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 09
Page:
1614-1618
Research Field:
Publishing date:

Info

Title:
Long non-coding RNA LINC00313 promotes proliferation and migration of lung cancer cells through ferroptosis resistance
Author(s):
HUANG Lin; LI Bin; HU Zuowei
Wuhan No.1 Hospital,Hubei Wuhan 430022,China.
Keywords:
lung cancer; LncRNA LINC00313; ferroptosis
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2023.09.007
Abstract:
Objective：To investigate the mechanism of long non-coding RNA(LncRNA) LINC00313 in promoting the proliferation and metastasis of lung cancer cells.Methods:Lung cancer cell lines A549,H466,H1299 and H522 were selected,and the expression of LINC00313 in lung cancer cells was detected by real-time PCR.Transwell test and scratch test were used to detect the invasion and migration of lung cancer cells.The effect of excess GPX4 on proliferation and apoptosis of lung cancer cells was detected by CCK-8 assay and flow cytometry after knockout of LINC00313.To investigate the relationship between LINC00313 and ferroptosis in lung cancer cells by detecting iron levels and ROS content in Erastin or RSL3 treated A549 cells after LINC00313 gene knockout.Results:Compared with normal cells,the expression of LINC00313 in lung cancer cells was significantly increased(P＜0.05).Phenotypic experiments showed that LINC00313 promoted the proliferation of lung cancer and inhibited its apoptosis.The results of Transwell assay and scratch assay showed that LINC00313 could promote the invasion and migration of lung cancer cells.GPX4 overexpression reversed the decreased proliferation and increased apoptosis induced by LINC00313 knockdown.In Erastin or RSL-3 treated A549 cells,the levels of iron and Fe2+ in the cells decreased after LINC00313 knockdown,and the production of lipid ROS decreased significantly.Conclusion:The expression of LINC00313 is significantly increased in lung cancer cells.Overexpression of LINC00313 can promote the invasion and migration of lung cancer cells,and the mechanism may be related to the promotion of ferroptosis resistance in lung cancer cells,thus leading to the occurrence and metastasis of lung cancer.

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Memo

Memo:
湖北省武汉市卫生健康委员会医学科研青年项目(编号：WZ20Q04)
Last Update: 2023-03-30