|Table of Contents|

Zishen Gusui Decoction induces apoptosis of colorectal cancer HCT-116 cells by regulating p38MAPK/p53 signaling pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 09
Page:
1608-1613
Research Field:
Publishing date:

Info

Title:
Zishen Gusui Decoction induces apoptosis of colorectal cancer HCT-116 cells by regulating p38MAPK/p53 signaling pathway
Author(s):
WU Ting; ZHANG Yunfang; QIU Xiaotang; CHEN Xuewu; JIANG Jingwen; XING Mingyuan; SU Yihua
Department of Oncology,Hainan Hospital of Traditional Chinese Medicine,Hainan Haikou 570203,Chi
Keywords:
Zishen Gusui Decoction; colorectal cancer; cell apoptosis; p38MAPK/p53 signaling pathway
PACS:
R735.3+5
DOI:
10.3969/j.issn.1672-4992.2023.09.006
Abstract:
Objective：To investigate the effect of Zishen Gusui Decoction on apoptosis of colorectal cancer HCT-116 cells and its molecular mechanism.Methods:Zishen Gushu Decoction containing serum was prepared.Firstly,HCT-116 cells were treated with different volume fraction of Zishen Gusui Decoction,and the proliferation activity of HCT-116 cells was detected by methyl thiazolyl-tetrazolium(MTT).Then HCT-116 cells were divided into blank group,Zishen Gushu Decoction low,medium and high dose groups,SB203580(p38MAPK inhibitor) group and Zishen Gushu Decoction high dose+SB203580 group.After the intervention with the corresponding drugs,Hoechst 33258 staining was used to observe the morphological changes of cell apoptosis.Annexin V-FITC flow cytometry was used to determine the apoptosis rate of the cells.Western blot was used to detect the protein expression levels of p38MAPK,phosphorylated(p)-p38MAPK(Thr180/Tyr182),mouse dimicrosomal 2(MDM2),p53,Cleaved caspase-3,Bax and Bcl-2.Results:Zishen Gusui Decoction could inhibit the proliferation of HCT-116 cells in a time-dependent and concentration-dependent manner.Treatment with different concentrations of Zishen Gusui Decoction could induce apoptotic morphological changes of HCT-116 cells such as nuclear aggregation,shrinkage or fragmentation,promote cell apoptosis,up-regulate the protein expression levels of p-p38MAPK,p53,Bax and Cleaved caspase-3 and down-regulate the protein expression levels of MDM2 and Bcl-2.However,SB203580 inhibited the apoptosis of HCT-116 cells and the activation of p38MAPK/p53 signaling pathway induced by Zishen Gushu Decoction.Conclusion:Zishen Gusui Decoction can induce apoptosis of HCT-116 cells by activating p38MAPK/p53 signaling pathway.

References:

[1] 武爱文,李英杰.结直肠癌新辅助免疫治疗的应用现状以及争议和挑战［J］.中华胃肠外志,2022,25(3):185-192. WU AW,LI YJ.Current status,controversy and challenge in the neoadjuvant immunotherapy of colorectal cancer［J］.Chin J Gastrointest Surg,2022,25(3):185-192.
[2] LIN S,AN X,GUO Y,et al.Meta-analysis of astragalus-containing traditional chinese medicine combined with chemotherapy for colorectal cancer:efficacy and safety to tumor response［J］.Front Oncol,2019,9:749.
[3] ZHANG Y,JIA Y,FANG Y,et al.Chinese medicine treatment of intestinalcancer diarrhea［J］.Front Med Sci Res,2021,3(6):11-14.
[4] 吴亭.滋肾固髓汤结合化疗在中期结直肠癌术后的短期临床观察［D］.广州:中医药大学,2019. WU T.Short-term clinical observation of Zishen Gusui Decoction combined with mFOLFOX6 chemotherapeutic regimen affects the mid-term colorectal cancer after operation［D］.Guangzhou:University of Chinese Medicine,2019.
[5] HE H,QIAO K,WANG C,et al.Hydrazinocurcumin induces apoptosis of hepatocellular carcinoma cells through the p38MAPK pathway［J］.Clin Transl Sci,2021,14(5):2075-2084.
[6] KHAJEH E,RASMI Y,KHERADMAND F,et al.Crocetin suppresses the growth and migration in HCT-116 human colorectal cancer cells by activating the p-38 MAPK signaling pathway［J］.Res Pharm Sci,2020,15(6):592-601.
[7]TANG TJ,WANG X,ZUO MY,et al.Protective effect of Linggui Zhugantang medicated serum against H2O2-induced injury in H9c2 cells by regulating PI3K/Akt signaling pathway［J］.Chinese Journal of Experimental Traditional Medical Formulae,2022,28(13):1-9.
[8] SUNG H,FERLAY J,SIEGEL RL,et al.Global cancer statistics 2020:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries［J］.CA Cancer J Clin,2021,71(3):209-249.
[9] GANESH K,STADLER ZK,CERCEK A,et al.Immunotherapy in colorectal cancer:rationale,challenges and potential［J］.Nat Rev Gastroenterol Hepatol,2019,16(6):361-375.
[10] ZHAO LN,YANG YQ,WANG WW,et al.The effects of traditional Chinese medicine combined with chemotherapy on immune function and quality of life in patients with non-small cell lung cancer:A protocol for systematic review and meta-analysis［J］.Medicine(Baltimore),2020,99(45):e22859.
[11] DUENSING TD,WATSON SR.Assessment of apoptosis(programmed cell death) by flow cytometry［J］.Cold Spring Harb Protoc,2018(1):38-40.
[12]黄智超,李国峰,郎一帆,等.中药诱导肺癌细胞凋亡机制的研究进展［J］.中国实验方剂学杂志,2021,27(23):226-236. HUANG ZC,LI GF,LANG YF,et al.Mechanism of chinese medicine in induction of apoptosis of lung cancer cells:a review［J］.Chinese Journal of Experimental Traditional Medical Formulae,2021, 27(23):226-236.
[13] 徐丽媛,窦露,靳烨,等.细胞凋亡及其与肌纤维的关联机制研究进展［J］.动物营养学报,2022,34(5):2847-2855. XU LY,DOU L,JIN Y,et al.Research progress of appoptosis and its correlation mechanism with muscle fibers［J］.Chinese Journal of Animal Nutrition,2022,34(5):2847-2855.
[14] 林思,朱华,秦慧真,等.对叶百部总生物碱对人肝癌SMMC-7721细胞凋亡及Bcl-2,Bax和cleaved Caspase-3蛋白表达的影响［J］.中国实验方剂学杂志,2021,27(19):73-79. LIN S,ZHU H,QIN HZ,et al.Effect of stemona tuberosaalkaloids on apoptosis of SMMC-7721 cells and expression of Bcl-2，Bax，and Cleaved Caspase-3［J］.Chinese Journal of Experimental Traditional Medical Formulae,2021,27(19):73-79.
[15] 倪鹏，宋岩，黄宝俊，等.结直肠癌微环境诱导腹膜间皮细胞凋亡的体外实验研究［J］.现代肿瘤医学，2020,28(23):4061－4065. NI P,SONG Y,HUANG BJ,et al.Colon cancer microenvironment promotes apoptosis of peritoneal mesothelial cells［J］.Modern Oncology,2020,28(23):4061－4065. ［16］ ZENG Z,ZHANG H,WANG X,et al.Salvianolic acid B suppresses cell proliferation and induces apoptosis in osteosarcoma through p38-mediated reactive oxygen species generation［J］.Oncol Lett, 2018,15(2):2679-2685.
[17] KIRIMTAY K,SELCUK E,KELLE D,et al.p53 regulates katanin-p60 promoter in HCT 116cells［J］.Gene,2020,727:144241.
[18] 唐亚纯,许武军,陈仙,等.抑制p38MAPK信号通路对尿源性脓毒血症兔肾组织细胞凋亡的影响及其作用机制［J］.安徽医科大学学报,2021,56(9):1417-1423. TANG YC,XU WJ,CHEN X,et al.Effect of inhibiting p38MAPK signaling pathway on apoptosis of renal tissue in rabbits with urosepsis and its mechanism［J］.Acta Universitatis Medicinalis Anhui, 2021,56(9):1417-1423.
[19] HE H,QIAO K,WANG C,et al.Hydrazinocurcumin induces apoptosis of hepatocellular carcinoma cells through the p38 MAPK pathway［J］.Clin Transl Sci,2021,14(5):2075-2084.
[20] SONG N,MA J,HU W,et al.Lappaconitine hydrochloride inhibits proliferation and induces apoptosis in human colon cancer HCT-116 cells via mitochondrial and MAPK pathway［J］.Acta Histochem,2021,123(5):151736.
[21] 仇雅岚,高静东,刘敏,等.基于网络药理学的健脾解毒方抗结直肠癌的作用机制研究［J］.南京中医药大学学报,2022,38(2):136-146. QIU YL,GAO JD,LIU M,et al.Study on the anti-colorectal cancer mechanism of Jianpi Jiedu Decoction based on network pharmacology［J］.J Nanjing Univ Tradit Chin Med,2022,38(2):136-146.
[22] 张东升,周方正,王小聪,等.CDX1对结直肠癌细胞增殖凋亡及p38MAPK磷酸化水平的影响 ［J］.现代肿瘤医学,2018,26(17):2665-2669. ZHANG DS,ZHOU FZ,WANG XC,et al.Effect of CDXl on the proliferation,apoptosis and the level of p38 MAPK phosphorylation in colorectaI cancer cells［J］.Modern Oncology,2018,26(17): 2665-2669.
[23] 叶帅,季华,赵晓彤,等.全长脂联素在高糖环境下对结肠癌细胞增殖与凋亡的影响［J］.安徽医科大学学报,2018,53(12):1879-1883. YE S,JI H,ZHAO XT,et al.Research on the effects of full length adiponectin on the proliferation andapoptosis of colon cancer cells in the high glucose environment［J］.Acta Universitatis Medicinalis Anhui,2018,53(12):1879-1883.

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Memo:
海南省自然科学基金委员会基础与应用基础研究计划（编号：821MS0837）
Last Update: 2023-03-30