|Table of Contents|

Study on the function and mechanism of lung cancer cells exosomal miR-24-3p regulating the anti-tumor of CD8+ T cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 09
Page:
1601-1607
Research Field:
Publishing date:

Info

Title:
Study on the function and mechanism of lung cancer cells exosomal miR-24-3p regulating the anti-tumor of CD8+ T cells
Author(s):
SONG Minglei1; CAO Fumin1; XING Xiaoying2; GAO Liping1; JING Hongjia3
1.Department of Thoracic Surgery,the Fourth Hospital of Hebei Medical University,Hebei Shijiazhuang 050011,China;2.General Medical Department,the Second Hospital of Hebei Medical University,Hebei Shijiazhuang 050011,China;3.Department of Cardiothoracic Surgery,Chengde Central Hospital,Hebei Chengde 067000，China.
Keywords:
lung cancer; exosomes; miR-24-3p; CD8+T cells
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2023.09.005
Abstract:
Objective：To explore the function and mechanism of lung cancer cells exosomal miR-24-3p on the anti-tumor function of CD8+T cells.Methods:16HBE exo,A549 exo,H522 exo,H460 exo,miR-NC exo,miR-24-3p exo and PBS were co-incubated with CD8+T cells(16HBE exo group,A549 exo group,H522 exo group,H460 exo group,miR-NC exo group,miR-24-3p exo group,PBS group),CD8+T was co-incubated with miR-24-3p exo and transfected into FGF11 plasmid(miR-24-3p exo+FGF11 group).The proliferation ability of CD8+T cells was detected by CCK8 method.The dual-luciferase reporter gene experiment verified the targeting relationship between miR-24-3p and FGF11.The above groups of CD8+T cells and A549 cells were co-incubated for 4 h at a ratio of 20∶1(16HBE exo/CD8+T group,A549 exo/CD8+T group,H522 exo/CD8+T group,H460 exo/CD8+T group,miR-NC exo/CD8+T group,miR-24-3p exo/CD8+T group,PBS/CD8+T group,miR-24-3p exo+FGF11/CD8+T group).Elisa was used to detect the concentrations of INF-γ and IL-2 in the supernatant,and the killing ability of CD8+T cells in each group was detected by LDH method.Results:Compared with the PBS group,the proliferation ability of CD8+T cells in the A549 exo group,H522 exo group and H460 exo group was significantly decreased(P＜0.05).The concentrations of INF-γ and IL-2 in the supernatant of A549 exo/CD8+T group,H522 exo/CD8+T group and H460 exo/CD8+T group were significantly decreased(P＜0.001),and the killing ability of CD8+T cell was also significantly decreased(P＜0.001).The results of dual fluorescein reporter gene assay showed that FGF11 was the target gene of miR-24-3p.The proliferation ability of CD8+T cells in miR-24-3p exo group was significantly lower than that in miR-NC exo group,and the proliferation ability of CD8+T cells in miR-24-3p exo+FGF11 group was significantly higher than that in miR-24-3p exo group(P＜0.05).The concentrations of INF-γ and IL-2 in the supernatant and CD8+T cell killing ability of the miR-24-3p exo/CD8+T group were significantly lower than those of the miR-NC exo/CD8+T group(P＜0.001),and the concentrations of INF-γ and IL-2 in the supernatant and the CD8+T cell killing ability of the miR-24-3p exo+FGF11/CD8+T group were significantly higher than those in miR-24-3p exo/CD8+T group(P＜0.001).Conclusion:Exosomal miR-24-3p in lung cancer cells can inhibit the anti-tumor function of CD8+T cells by targeting FGF11.

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Last Update: 2023-03-30