|Table of Contents|

miR-144-5p regulates the proliferation,migration and invasion of papillary thyroid cancer cells by targeting ACTG1 and STAT1

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 09
Page:
1595-1601
Research Field:
Publishing date:

Info

Title:
miR-144-5p regulates the proliferation,migration and invasion of papillary thyroid cancer cells by targeting ACTG1 and STAT1
Author(s):
GAO Shengyu1; DING Chenglong2; WEI Weiwei1
1.Department of General Surgery;2.Department of Pathology,the First Affiliated Hospital of Jiamusi University,Heilongjiang Jiamusi 154002,China.
Keywords:
miR-144-5p; ACTG1; STAT; papillary thyroid carcinoma; bioinformatics database
PACS:
R736.1
DOI:
10.3969/j.issn.1672-4992.2023.09.004
Abstract:
Objective：To investigate the expression trend of miR-144-5p in papillary thyroid carcinoma(PTC) cells and its effect on the proliferation,invasion and migration of PTC cell lines and related molecular mechanisms.Methods:Quantitative real-time polymerase chain reaction(qRT-PCR) was used to detect the expression of miR-144-5p in human PTC cell lines TPC-1 and BCPAP,and human normal thyroid follicular cell line Nthy-ori 3-1.miR-144-5p-mimics,miR-144-5p-inhibitor,inhibitor-NC and mimics-NC were transfected into TPC-1 cells by LipofectaminTM2000,respectively.The cells were divided into 4 groups:Negative control group(mimics-NC group),overexpression group(mimics-144-5p group),negative control group(inhibitor NC group) and inhibitor-144-5p group(inhibitor-144-5p group).The cell proliferation,invasion and migration of the four groups were detected by CCK-8 growth curve,chamber invasion test and scratch test.ENCORI database was used to verify the expression trend of miR-144-5p in TCGA.The downstream target genes of miR-144-5p were predicted by online database.The target gene-protein interaction network was obtained by STING.The genes with Degree value Top10 were obtained by Cytascap plugin CytoHubba.GEPIA database was used to detect the expression trends of ACTG1 and STAT1 in TCGA database.ACTG1 and STAT1 protein expression levels were verified by HPA database.qRT-PCR and TaretScan online database were used to verify the regulatory relationship between miR-144-5p and ACTG1 and STAT1.Results:The expression trend of miR-144-5p in papillary thyroid carcinoma(PTC) cell lines was significantly lower than that in human normal thyroid follicular epithelium cell line Nthy-ori 3-1.The proliferation activity,invasion and migration ability of TPC-1 cells were significantly decreased after the upregulation of miR-144-5p,while the inhibition of miR-144-5p had the opposite effect.The online database predicted 584 downstream target genes of miR-144-5p.The target gene-protein interaction network was obtained by STING.The genes with Degree value of Top10 were obtained by Cytascap plug-in CytoHubba.The online database ENCORI verified that ACTG1 and STAT1 might be the downstream target genes of miR-144-5p.qRT-PCR verified the negative regulatory relationship between miR-144-5p and ACTG1 and STAT1.Conclusion:miR-144-5p is lowly expressed in PTC cells.Overexpression or knockdown of miR144-5p may affect the proliferation,invasion and migration ability of PTC cells by regulating ACTG1 and STAT1.

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Memo:
黑龙江省卫生健康委课题(编号：2020-328)
Last Update: 2023-03-30