|Table of Contents|

LncRNA DARS-AS1 promotes osteosarcoma cell proliferation and inhibits apoptosis by targeting miR-758-3p

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 09
Page:
1589-1594
Research Field:
Publishing date:

Info

Title:
LncRNA DARS-AS1 promotes osteosarcoma cell proliferation and inhibits apoptosis by targeting miR-758-3p
Author(s):
ZHANG Bo; HU Lingyun; GOU Lin; JIA Min; LI Yongning; LUO Xuwei; LI Wei
Department of Orthopedics,Nanchong Central Hospital,Sichuan Nanchong 637000,China.
Keywords:
osteosarcoma; LncRNA DARS-AS1; miR-758-3p; cell proliferation; cell apoptosis
PACS:
R738.1
DOI:
10.3969/j.issn.1672-4992.2023.09.003
Abstract:
Objective：To explore the effect of LncRNA DARS-AS1 on the proliferation and apoptosis of osteosarcoma cells and its possible mechanism.Methods:The expression of LncRNA DARS-AS1 and miR-758-3p in osteosarcoma tissues,adjacent tissues,human osteoblasts hFOB 1.19,human osteosarcoma cells Saos2,U2OS,and HOS were detected by qRT-PCR.Transfection of Saos2 cells was divided into si-NC group,si-LncRNA DARS-AS1 group,miR-NC group,miR-758-3p group,si-LncRNA DARS-AS1+anti-miR- NC group,si-LncRNA DARS-AS1+anti-miR-758-3p group.MTT method and flow cytometry were used to detect cell proliferation and apoptosis.The dual luciferase reporter experiment was used to detect the targeted regulation relationship between LncRNA DARS-AS1 and miR-758-3p.Results:Compared with adjacent tissues,the expression of LncRNA DARS-AS1 in osteosarcoma tissue was increased(P＜0.05),while the expression of miR-758-3p was decreased(P＜0.05).Compared with hFOB 1.19 cells,the expression of LncRNA DARS-AS1 in Saos2,U2OS,HOS cells was increased(P＜0.05),while the expression of miR-758-3p was decreased(P＜0.05).Compared with the si-NC group,the cell viability in the si-LncRNA DARS-AS1 group was decreased(P＜0.05),while the apoptosis rate was increased(P＜0.05).Compared with the miR-NC group,the cell viability in the miR-758-3p group was decreased(P＜0.05),while the apoptosis rate was increased(P＜0.05).LncRNA DARS-AS1 could target miR-758-3p.Compared with the si-LncRNA DARS-AS1+anti-miR-NC group,the cell viability in the si-LncRNA DARS-AS1+anti-miR-758-3p group was increased(P＜0.05),while the cell apoptosis rate was decreased(P＜0.05).Conclusion:Interfering with the expression of LncRNA DARS-AS1 could inhibit the proliferation of osteosarcoma cells and induce apoptosis by targeting the regulation of miR-758-3p expression.

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Last Update: 2023-03-30