|Table of Contents|

CXCR4 regulates the migration of head and neck squamous cell carcinoma cells through integrinαVβ3

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 09
Page:
1584-1588
Research Field:
Publishing date:

Info

Title:
CXCR4 regulates the migration of head and neck squamous cell carcinoma cells through integrinαVβ3
Author(s):
CHANG Yanyan; LI Peng; MENG Jinping; CHEN Yanyan
Department of Head and Neck Thyroid,Henan Cancer Hospital,Henan Zhengzhou 450000,China.
Keywords:
CXCR4; integrinαVβ3; head and neck squamous cell carcinoma; cell migration
PACS:
R730.267
DOI:
10.3969/j.issn.1672-4992.2023.09.002
Abstract:
Objective：To study the molecular mechanism of CXCR4-integrinαVβ3 mediating migration of head and neck squamous cell carcinoma cells.Methods:Head and neck squamous cell carcinoma cell line HN-5 was used to construct CXCR4 overexpression cell line by lentivirus transfection,and the gene expression was observed by fluorescence microscope.CXCR4-siRNA and integrinαVβ3-siRNA were transfected into HN-5 cells and CXCR4-overexpressed HN-5 cells by liposome method,respectively.The migration ability of HN-5 cells was detected by Transwell chamber assay.The expressions of CXCR4,integrinαVβ3,matrix metalloproteinase-9(MMP-9),matrix metalloproteinase-2(MMP-2) and adhesion spot kinase(FAK) -non-receptor tyrosine protein kinase(SRC)-extracellular signal-regulated kinase(ERK) signaling pathway related proteins were detected by Western blot analysis.Results:Overexpression of CXCR4 up-regulated the expression of integrinαVβ3 in HN-5 cells,while inhibition of CXCR4 down-regulated the expression of integrinαVβ3.CXCR4 overexpression can regulate MMP-2 and MMP-9 to promote cancer cell migration and activate FAK-SRC-ERK signaling pathway.Inhibition of integrinαVβ3 expression significantly reversed the above effects of CXCR4 overexpression on HN-5 cells.Conclusion:CXCR4 overexpression can promote the migration of head and neck squamous cell carcinoma cells through integrinαVβ3,and its mechanism may be related to the activation of FAK-SRC-ERK pathway.

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河南省医学科技攻关计划项目（编号：2018020488）
Last Update: 2023-03-30