|Table of Contents|

miR-497-5p regulates migration and invasion of esophageal squamous cell carcinoma through HMGA2

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 08
Page:
1434-1439
Research Field:
Publishing date:

Info

Title:
miR-497-5p regulates migration and invasion of esophageal squamous cell carcinoma through HMGA2
Author(s):
LIU Huimin; LIU Honghong; LIU Xiaoyan
Department of Gastroenterology,the Fifth Affiliated Hospital of Xinjiang Medical University,Xinjiang Urumqi 830011,China.
Keywords:
miR-497-5p; HMGA2; ESCC; migration and invasion
PACS:
R735.1
DOI:
10.3969/j.issn.1672-4992.2023.08.009
Abstract:
Objective:To investigate the effect of miR-497-5p on the migration and invasion of esophageal squamous cell carcinoma (ESCC) by high mobility group protein A2 (HMGA2).Methods:UALCAN and GEPIA databases were used to analyze the expression of miR-497-5p and HMGA2 mRNA in esophageal carcinoma (ESCA) and its control samples.The correlation between miR-497-5p and HMGA2 expression in ESCA samples was analyzed by starBase,a bioinformatics website,and the binding sites between them were predicted.The regulation effect of miR-497-5p on HMGA2 was confirmed by dual luciferase reporter assay.The effect of miR-497-5p on the migration and invasion of ESCC cells through HMGA2 was analyzed by Wound healing assay and Transwell assay.Results:The expression of miR-497-5p in ESCA was significantly lower than that in the control samples (P＜0.05),and the expression of HMGA2 mRNA in ESCA was significantly higher than that in the control samples (P＜0.05).miR-497-5p was negatively correlated with HMGA2 in ESCA samples.There were binding sites between miR-497-5p and HMGA2,and miR-497-5p directly regulated the target gene HMGA2.The migration and invasion ability of miR-497-5p mimic group were significantly lower than that of negative control (NC) group (P＜0.001).The migration and invasion ability of miR-497-5p mimic+HMGA2 group were significantly increased compared with mir-497-5p mimic group (P＜0.01).Conclusion:miR-497-5p regulates the migration and invasion of ESCC via HMGA2.

References:

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Memo

Memo:
新疆维吾尔自治区自然科学基金资助项目(编号:2021D01C451)
Last Update: 1900-01-01