|Table of Contents|

Influences of recombinant human endostatin combined with TACE on microvessel density and Notch pathway of rabbit model of VX-2 liver cancer

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 08
Page:
1428-1434
Research Field:
Publishing date:

Info

Title:
Influences of recombinant human endostatin combined with TACE on microvessel density and Notch pathway of rabbit model of VX-2 liver cancer
Author(s):
YANG Bo; LI Yuwei; PAN Feng; LIU Yuyu
Department of Interventional Medicine,Affiliated Hospital of Southwest Medical University,Sichuan Luzhou 646000,China.
Keywords:
recombinant human endostatin; transcatheter arterial chemoembolization; liver cancer; drosophila double wing margin nicked homologous gene (Notch) pathway; microvessel density
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2023.08.008
Abstract:
Objective:To investigate the regulatory effect of recombinant human endostatin (rhES) combined with transcatheter arterial chemoembolization (TACE) on drosophila double wing margin nicked homologous gene (Notch) pathway and its influence on microvessel density (MVD) of the rabbit model of VX-2 liver cancer.Methods:Fifty of sixty New Zealand white rabbits were randomly selected to establish the VX-2 liver cancer model by tumor implantation,and they were grouped into model group,TACE group,TACE+rhES group (TACE+rhES solution in 0.75 mg/kg),Notch activator jagged1 group (TACE+jagged1 solution in 13 ng/kg),TACE+rhES+jagged1 group (TACE+rhES solution in 0.75 mg/kg+jagged1 solution in 13 ng/kg),with 10 rabbits in each group,and the 10 rabbits without model were served as normal control group.After 2 weeks of treatment,CT imaging was performed to obtain the blood flow (BF),blood volume (BV) and capillary surface permeability area (PS) around the tumor of rabbits.The weight of rabbits was weighed,the rabbits were sacrificed and their livers were taken to calculate the liver index and tumor volume.Immunohistochemistry was used to detect the MVD of liver.Western blot was used to detect the relative protein expression levels of Notch,Notch ligand (jagged1),vascular endothelial growth factor (VEGF),and activated protein 1 (AP-1) in non-necrotic liver tissue around the tumor.Results:Compared with the normal control group,the rabbits in the model group had decreased diet,decreased activity and apathy,and the tumor nodules were dark red with pathological damages such as necrosis,liquefaction and cystic changes.The liver index,tumor volume,MVD,protein expressions of Notch,jagged1,VEGF and AP-1 were higher (P＜0.05),and the body weight and levels of BF,BV and PS were lower (P＜0.05).Compared with the model group,the tumor nodule in TACE group was milky white,and the tumor volume was obviously lower (P＜0.05),and there was no obvious difference in other indicators (P＞0.05).The degrees of mental depression and tumor necrosis in jagged1 group increased,and the liver index,tumor volume,MVD,protein expressions of Notch,jagged1,VEGF and AP-1 were higher (P＜0.05),and the body weight and levels of BF,BV and PS were lower (P＜0.05).The diet and spirit of rabbits in TACE+rhES group were improved,and the degree of tumor necrosis was reduced,the liver index,tumor volume,protein expressions of Notch,jagged1,VEGF and AP-1 were lower (P＜0.05),and the body weight and the levels of BF,BV and PS were higher (P＜0.05).Compared with those in TACE+rhES group,the above indexes in TACE+rhES+jagged1 group showed opposite trends,and the difference was statistically obvious (P＜0.05).Conclusion:The combined therapy of TACE+rhES can inhibit tumor angiogenesis in rabbit model of liver cancer by inhibiting the activation of Notch/jagged1 signaling pathway.

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Memo

Memo:
西南医科大学校级基金资助计划项目（编号：2017-ZRQN-141）
Last Update: 1900-01-01