|Table of Contents|

Overexpression of OMA1 induces apoptosis of lung adenocarcinoma A549 cells by promoting mitochondrial release of cytochrome C

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 08
Page:
1414-1419
Research Field:
Publishing date:

Info

Title:
Overexpression of OMA1 induces apoptosis of lung adenocarcinoma A549 cells by promoting mitochondrial release of cytochrome C
Author(s):
SUN Yongpan; LONG Qian; ZHOU Xiaojiang; KONG Demiao; LIU Bo; LU Zhansheng
Department of Thoracic Surgery，Guizhou Provincial People's Hospital,Guizhou Guiyang 550002,China.
Keywords:
lung adenocarcinoma; OMA1; cytochrome C; cell apoptosis; mitochondria
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2023.08.006
Abstract:
Objective:To investigate the expression level of OMA1 in lung adenocarcinoma and its effect on apoptosis of lung adenocarcinoma A549 cells and its mechanism.Methods:The cancerous tissues samples and para-cancerous tissues (≥5 cm from the tumor margin) were collected from 42 patients with pathologically confirmed lung adenocarcinoma stored in our hospital from January 2018 to October 2019.The expression of OMA1 in cancerous tissues and para-cancerous tissues was detected by immunohistochemistry.Human lung adenocarcinoma cell lines NCI-H2009,Calu-3,SPC-A-1,A549 and human normal lung epithelial cells BEAS-2B were cultured in vitro.The expression levels of OMA1 mRNA and protein in cells were detected by qRT-PCR and Western blotting.A549 cells were infected with OMA1 overexpression lentivirus and its no-load lentivirus,which were divided into OMA1 overexpression lentivirus infection group (OMA1 group) and no-load lentivirus infection group (Vector group),and blank control group (Blank group) was set.The cell proliferation activity was detected by MTT.The apoptosis level was detected by flow cytometry.The changes of mitochondrial membrane potential were detected by JC-10 staining.The expression level of OMA1 mRNA was detected by qRT-PCR.Western blotting was used to detect the expression levels of OMA1,cleaved caspase-3,OPA1,and Cyt C protein in mitochondria and cytoplasm of cells.Results:The expression level of OMA1 in cancerous tissues of lung adenocarcinoma patients was significantly lower than that in para-cancerous tissues (P＜0.05).The mRNA and protein expression levels of OMA1 in NCI-H2009,Calu-3,SPC-A-1 and A549 cells were significantly lower than those in BEAS-2B cells (P＜0.05).Compared with Blank group or Vector group,the mRNA and protein expression levels of OMA1,apoptosis rate,expression levels of cleaved caspase-3 protein and Cyt C protein in cytoplasm of OMA1 group were significantly increased (P＜0.05),while the cell proliferation activity,mitochondrial membrane potential,expression levels of OPA1 protein and Cyt C protein in mitochondria were significantly decreased (P＜0.05).Conclusion:OMA1 is low expressed in lung adenocarcinoma tissues and cell lines,and overexpression of OMA1 can induce apoptosis of lung adenocarcinoma A549 cells by down-regulating OPA1 protein expression,damaging mitochondrial membrane potential,and accelerating the release of mitochondrial Cyt C.

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贵州省科技厅科技计划项目（编号：黔科合基础【2019】1202）
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