|Table of Contents|

Differential expression of lncRNA NONHSAT070341 in cervical cancer and its effect on the biological characteristics of cervical cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 08
Page:
1391-1397
Research Field:
Publishing date:

Info

Title:
Differential expression of lncRNA NONHSAT070341 in cervical cancer and its effect on the biological characteristics of cervical cancer cells
Author(s):
WEI Yimiao1; WEN Songquan1; WANG Xiuting2; WANG Shuling3; BAI Ying1; LI Jiarong3; LI Qi1; ZHAO Weihong1
1.Department of Gynecology and Obstetrics,the Second Clinical Medical College of Shanxi Medical University,Shanxi Taiyuan 030001,China;2.Department of Biochemistry and Molecular Biology,Basic Medical College,Shanxi Medical University,Shanxi Taiyuan 030001,China;3.Department of Epidemiology,School of Public Health,Shanxi Medical University,Shanxi Taiyuan 030001,China.
Keywords:
cervical cancer; lncRNA; NONHSAT070341; proliferation; migration; invasion
PACS:
R737.33
DOI:
10.3969/j.issn.1672-4992.2023.08.003
Abstract:
Objective:To explore the expression of long noncoding RNA (lncRNA) NONHSAT070341 in cervical cancer tissues and cell lines SiHa and Caski and its effect on the biological functions of cervical cancer cells such as proliferation,migration,invasion,apoptosis and cell cycle distribution.Methods:A total of 90 patients with different stages of cervical lesions who underwent surgical resection in the department of gynecology and obstetrics,the Second Clinical Medical College of Shanxi Medical University from June 2021 to June 2022 were collected,and 30 cases of normal cervical tissue undergoing total hysterectomy due to uterine fibroids at the same time were used as control.The expression of lncRNA NONHSAT070341 in cervical cancer tissues and cell lines was detected by quantitative real-time polymerase chain reaction (qRT-PCR).The lncRNA NONHSAT070341 was knocked down by transient transfection of small interfering RNA (siRNA) fragment in SiHa and Caski cells,and the transfection efficiency was verified by qRT-PCR.The cell proliferation ability was detected by CCK-8 and plate colony formation assay.The cell cycle assay was used to detect the effect on the cycle distribution of cervical cancer cells.The scratch healing assay was used to evaluate the lateral migration ability of cells,and the Transwell assay was used to determine the longitudinal migration and invasion ability of cells.Flow cytometry analyzed cell apoptosis.Results:The lncRNA NONHSAT070341 was highly expressed in cervical cancer tissues and cells.NONHSAT070341 siRNA can reduce the expression of NONHSAT070341 in SiHa and Caski cells.After knocking down NONHSAT070341,the proliferation,migration and invasion abilities of cervical cancer cells were significantly reduced,and the cells were arrested in the G1 phase,influencing the distribution of cervical cancer cell cycle，whereas the rate of apoptosis of SiHa and Caski cells were enhanced.Conclusion:NONHSAT070341 can promote the proliferation,migration and invasion of cervical cancer cells,change the biological behavior of cervical cancer and be expected to become an emerging biomarker for the diagnosis of cervical cancer.

References:

[1] LI DJ,SHI J,JIN J,et al.Epidemiological trend of cervical cancer［J］.Chin J Oncol,2021,43(9):912-916.
[2] SUNG H,FERLAY J,SIEGEL RL,et al.Global cancer statistics 2020:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries［J］.CA Cancer J Clin,2021,71(3):209-249.
[3] EUN TJ,PERKINS RB.Screening for cervical cancer［J］.Med Clin North Am,2020,104(6):1063-1078.
[4] WANG R,PAN W,JIN L,et al.Human papillomavirus vaccine against cervical cancer:Opportunity and challenge［J］.Cancer Lett,2020,471(2):88-102.
[5] FANG Y,FULLWOOD MJ.Roles,functions,and mechanisms of long non-coding RNAs in cancer［J］.Genomics Proteomics Bioinformatics,2016,14(1):42-54.
[6] XING C,SUN SG,YUE ZQ,et al.Role of lncRNA LUCAT1 in cancer［J］.Biomed Pharmacother,2021,134(2):1-10.
[7] ZHAO Y,SUN H,WANG H.Long noncoding RNAs in DNA methylation:new players stepping into the old game［J］.Cell Biosci,2016,6(7):45-51.
[8] O' LEARY VB,HAIN S,MAUGG D,et al.Long non-coding RNA PARTICLE bridges histone and DNA methylation［J］.Sci Rep,2017,7(1):1-12.
[9] HAN P,CHANG CP.Long non-coding RNA and chromatin remodeling［J］.RNA Biol,2015,12(10):1094-1098.
[10]YOON JH,ABDELMOHSEN K,GOROSPE M.Functional interactions among microRNAs and long noncoding RNAs［J］.Semin Cell Dev Biol,2014,34(10):9-14.
[11]SHENG H,ZHANG J,MA Y,et al.lncRNA FBXL19-AS1 is a diagnosis biomarker for paediatric patients with acute myeloid leukemia［J］.J Gene Med,2021,23(3):1-6.
[12] HUANG X,SHI H,SHI X,et al.LncRNA FBXL19-AS1 promotes proliferation and metastasis of cervical cancer through upregulating COL1A1 as a sponge of miR-193a-5p［J］.J Biol Res (Thessalon),2021,28(1):20-34.
[13] YANG X,XIE Z,LEI X,et al.Long non-coding RNA GAS5 in human cancer［J］.Oncol Lett,2020,20(3):2587-2594.
[14] POLLMANN MARC,PARWARESCH REZA,ADAM-KLAGES SABINE,et al.Human EML4,a novel member of the EMAP family,is essential for microtubule formation［J］.Exp Cell Res,2006,312(17):3241-3251.
[15] TAKEUCHI K,SODA M,TOGASHI Y,et al.RET,ROS1 and ALK fusions in lung cancer［J］.Nature Medicine,2012,18(3):378-381.
[16] KARIMI ZARCHI M,BEHTASH N,CHITI Z,et al.Cervical cancer and HPV vaccines in developing countries［J］.Asian Pac J Cancer Prev,2009,10(6):969-974.
[17] YUAN Y,CAI X,SHEN F,et al.HPV post-infection microenvironment and cervical cancer［J］.Cancer Lett,2021,497(1):243-254.
[18] MARTIN CM,KEHOE L,SPILLANE CO,et al.Gene discovery in cervical cancer:towards diagnostic and therapeutic biomarkers［J］.Mol Diagn Ther,2007,11(5):277-290.
[19] LUAN Y,XIE B,WEI W.REST-repressed lncRNA NPPA-AS1 regulates cervical cancer progression by modulating miR-302e/DKK1/Wnt/β-catenin signaling pathway［J］.J Cell Biochem,2021,122(1):16-28.
[20]NIKPAYAM E,TASHARROFI B,SARRAFZADEH S,et al.The role of long non-coding RNAs in ovarian cancer［J］.Iran Biomed J,2017,21(1):3-15.
[21] TORNESELLO ML,FARAONIO R,BUONAGURO L,et al.The role of microRNAs,long non-coding RNAs,and circular RNAs in cervical cancer［J］.Front Oncol,2020,10(2):1-16.
[22] SCHMITT AM,CHANG HY.Long noncoding RNAs in cancer pathways［J］.Cancer Cell,2016,29(4):452-463.
[23] FATICA A,BOZZONI I.Long non-coding RNAs:new players in cell differentiation and development［J］.Nat Rev Genet,2014,15(1):7-21.
[24] HU YL,ZHANG YX,LIU N,et al.LncRNA MIR503HG regulated cell viability,metastasis and apoptosis of cervical cancer via miR-191/CEBPB axis［J］.Eur Rev Med Pharmacol Sci,2021,25(8):3200-3210.

Memo

Memo:
National Natural Science Foundation of China(No.81702583）；国家自然科学基金（编号：81702583）；中国博士后科学基金（编号：2019M651072）;山西省优秀青年基金项目（编号：201901D211506）；山西省回国留学人员科研教研资助项目（编号：2022-195）
Last Update: 1900-01-01