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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2023 07

Page:

1231-1237

Research Field:

Publishing date:

Info

Title:

Expression of LINC00659 in gastric cancer tissues and its effect on invasion,migration and autophagy of human gastric cancer cells

Author(s):

LI Bing¹; TAO Yuejia¹; ZHAO Yuan¹; XU Yuanyi¹; HUANG Yunning²; LI Zhaojun³; GAO Wenwei⁴

1.Department of Pathology,School of Basic Medical Sciences,Ningxia Medical University,Ningxia Yinchuan 750004,China;2.Department of Gastrointestinal,People's Hospital of Ningxia Hui Autonomous Region,Ningxia Yinchuan 750002,China;3.School of Nursing,Ningxia Medical University,Ningxia Yinchuan 750004,China;4.Department of Pathogenic Biology and Immunology,Ningxia Medical University,Ningxia Yinchuan 750004,China.

Keywords:

gastric cancer; LINC00659; invasion; migration; autophagy

PACS:

R73-3

DOI:

10.3969/j.issn.1672-4992.2023.07.009

Abstract:

Objective:To detect the expression of LINC00659 in gastric cancer tissues and investigate the effects of LINC00659 on invasion,migration and autophagy of human gastric cancer cells AGS and HGC-27.Methods:The tumor tissue and paracancer tissue samples of 17 patients with gastric cancer were collected from the People's Hospital of Ningxia Hui Autonomous Region from January to November 2020.The expression of LINC00659 in human gastric cancer tissue and paracancer tissue was detected by qRT-PCR,and the expression of autophagy-asociated protein LC3 and p62 in human gastric cancer and paracancer tissue was detected by Western blot and immunohistochemistry.Knockdown and overexpression LINC00659 gastric cancer stable cells were constructed in AGS and HGC-27 cell lines,respectively,and divided into Vector group,sh-LINC00659 group,pEX-3 group and LINC00659 group.After After 48 h lentivirus transfection,transfection efficiency was detected by inverted fluorescence microscope and qRT-PCR.Transwell,scratch assay was used to detect the invasion and migration of cells in each group.Western blot,immunofluorescence was used to detect the relative expression levels of autophagy related protein LC3,p62 in each group.Results:The expression of LINC00659 and autophagy-associated protein LC3 in gastric cancer tissues was significantly higher than that in adjacent tissues,while the expression of autophagy-associated protein p62 in gastric cancer tissues was lower than that in adjacent tissues(all P<0.05).After transfection with lentivirus,the relative expression level of LINC00659 in AGS,HCC-27 cells was significantly decreased in the knockdown group and significantly increased in the overexpression group compared knockdown with the no-load group(all P<0.01).Transwell,scratch test results showed that compared with the control group,the invasion and migration of AGS knockdown cells were significantly inhibited,while the invasion and migration of HGC-27 overexpressed cells were significantly enhanced(all P<0.05).Western blot,immunofluorescence results showed that the expression of autophagy related protein LC3 was down-regulated in the AGS knockout group,while the expression of p62 was significantly increased,and the expression level of autophagy related protein LC3,p62 in the HGC-27 overexpression group was completely opposite to that in the knockout group（all P＜0.05).Conclusion:LINC00659 is highly expressed in gastric cancer tissues and can promote the invasion,migration and autophagy of human gastric cancer cells.

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Memo

Memo:

宁夏自然科学基金（编号：2020AAC03184）;宁夏重点研发项目（编号：2019BEG03007）;宁夏高校一流学科二期建设—重点培育学科项目(编号：NYHLZD03)

Common functions

Last Update: 2023-02-28