|Table of Contents|

Study on the effects of （-）gossypol in inducing apoptosis of human prostate cancer PC-3 cells in vitro

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2011 02
Page:
251-0254
Research Field:
Publishing date:

Info

Title:
Study on the effects of （-）gossypol in inducing apoptosis of human prostate cancer PC-3 cells in vitro
Author(s):
WANG Wen-ting1; HUANG Xiao-feng2; MU Shi-jie1; HU Xing-bin1; ZHAN Yong-hua3; XIA Ai-jun1; LIU Zhi-xin1; AN Qun-xing1; CHEN Rui1; ZHANG Xian-qing1
1Department of Blood Bank,Xijing Hospital , Fourth Military Medical University, Xi'an 710032,China；2Central Laboratory, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China;3School of Life Sciences and Technology, Xidian University, Xi'an 710071, China.
Keywords:
(-)gossypol; PC-3 cells; apoptosis; Bcl-2; Bak
PACS:
R73-36+1
DOI:
-
Abstract:
Objective：To investigate the effect of (-)gossypol on the proliferation and apoptosis in human prostate cancer PC-3 cells in vitro. Methods: The cell proliferation was evaluated by methyl thiazolyl tetrazolium (MTT)assay. Cell ultramicro-structure was studied by transmission electron microscope. The morphology of apoptotic cells was observed by TUNEL staining , cell cycle and apoptosis were detected by flow cytometry(FCM).The Bcl-2 and Bak mRNA level were measured by semiquantitative RT-PCR. Rusults: (-)Gossypol significantly inhibited the proliferation of PC-3 cells in a dose-and time-dependent manner. The PC-3 cells were arrested in G0/G1 phase and showed typical apoptosis characteristics in FCM and transmission electron microscope analysis.(-)Gossypol down-regulated Bcl-2 mRNA and up-regulated Bak mRNA level. Conclusion: (-)Gossypol can induce apoptosis of human prostate cancer PC-3 cells in vitro and the main reason may be related with the up-regulation of Bak mRNA and the down-regulation of Bcl-2 mRNA.

References:

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