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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2024 10

Page:

1824-1831

Research Field:

Publishing date:

Info

Title:

Analysis of serum differential proteins in patients with bone metastases and non-bone metastases in lung adenocarcinoma based on 4D-DIA proteomics

Author(s):

LI Maochen¹; 2; LAI Xueting²; ZHANG Hongxia¹; 2; WANG Bu²; ZHANG Zhihua²; ZHANG Xiulong²

1.Graduate School of Hebei North University，Hebei Zhangjiakou 075000，China;2.The First Affiliated Hospital of Hebei North University，Hebei Zhangjiakou 075000，China.

Keywords:

lung adenocarcinoma; bone metastases; proteomics; novel protein markers

PACS:

R734.2

DOI:

10.3969/j.issn.1672-4992.2024.10.011

Abstract:

Objective：To find the differential proteins between lung adenocarcinoma bone metastasis and adenocarcinoma non-bone metastasis based on deep blood 4D-DIA proteomic analysis,so as to provide a basis for diagnosis and treatment of lung adenocarcinoma bone metastasis.Methods:Serum proteomics analysis was performed in 6 patients with lung adenocarcinoma non-bone metastases (LUADNBM) and 6 patients with lung adenocarcinoma bone metastasis (LUADBM) using 4D-DIA proteomics.The expression fold (fold change,FC) was >1.5-fold (up-regulated by more than 1.5-fold or down-regulated by less than 0.67-fold) and P value<0.05 (T-test or other) was used as the standard to obtain the number of up-regulated and down-regulated proteins between the comparison groups.Gene set enrichment analysis (GSEA) was performed on quantifiable proteins between different comparison groups.Principal component analysis (PCA),gene ontology (GO) analysis and KEGG （Kyoto encyclopedia of genes and genomes） pathway database were performed on the String website and R language.Results:A total of 2 585 quantifiable proteins were found between the LUADNBM group and the LUADBM group,including 18 differential proteins.The results of GSEA showed that the quantified protein was mainly enriched in the eukaryotic translation extension stage and the main translation mechanism pathway of SARS-CoV-2 (P＜0.05).The results of PCA showed that the differential protein could clearly distinguish LUADNBM from LUADBM patients.The results of GO analysis showed that the differential proteins were mainly enriched in the lumen of the Golgi canal and extracellular matrix,and played an important role in the biosynthesis and metabolism of cutaneous sulfate proteoglycans and the catabolism and biosynthesis of chondroitin sulfate.The results of KEGG showed that the up-regulated proteins were mainly enriched in the pathways of proximal tubular bicarbonate recovery,bile secretion,N-glycan biosynthesis,insulin secretion,and aldosterone synthesis and secretion.Down-regulated proteins were mainly enriched in neurodegenerative diseases,such as Alzheimer's disease,Huntington's disease,Parkinson's disease,spinocerebellar ataxia,etc.Conclusion:There are significant differences between bone metastasis and non-bone metastasis histones in lung adenocarcinoma,and the discovery of novel protein markers provides new clues for early screening and diagnosis of bone metastasis.

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河北省医学科学研究课题计划(编号：20240476)

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