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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2024 08

Page:

1387-1392

Research Field:

Publishing date:

Info

Title:

ILF2 promotes the proliferation of non-small cell lung cancer cells via regulation of mitochondrial homeostasis in vivo and in vitro

Author(s):

HE Lihua¹; 2; ZHAO Meng³; LIU Shuye¹; 2; ZHU Yu¹; 2

1.The Third Central Clinical College of Tianjin Medical University,Tianjin 300170,China;2.Department of Clinical Laboratory,the Third Central Hospital of Tianjin,Tianjin Key Laboratory of Extracorporeal Life Support for Critical Diseases,Artificial Cell Engineering Technology Research Center of Tianjin,Tianjin Institute of Hepatobiliary Disease,Tianjin 300170,China;3.Department of Clinical Laboratory,Tianjin Medical University Cancer Institute and Hospital,Tianjin 300060,China.

Keywords:

interleukin enhancer binding factor 2; non-small cell lung cancer; mitochondrial homeostasis

PACS:

R734.2

DOI:

10.3969/j.issn.1672-4992.2024.08.003

Abstract:

Objective:To investigate the role of interleukin enhancer binding factor 2 (ILF2) in the proliferation of non-small cell lung cancer cells and to explore its mechanism.Methods:Lung cancer and adjacent normal tissue samples were obtained from non-small cell lung cancer patients (n=15) in a hospital from March 2016 to June 2022.The expression of ILF2 in A549 and H1299 cell lines of non-small cell lung cancer was inhibited by shRNA assay.The expression of ILF2 mRNA and protein in tissues and cells was detected by qRT-PCR,immunohistochemistry and Western blot assay.The proliferation of A549 and H1299 cells in vitro was analyzed by clone formation and BrdU assay,and the expression of Ki67 and cleaved caspase 3 was observed by immunohistochemistry after subcutaneously injection of lung cancer cells A549 in athymic nude mice.The mitochondrial homeostasis was observed by mtDNA,mitochondrial mass and mitochondrial membrane potential in A549 and H1299 cells.Results:Compared with the adjacent group,the expression of ILF2 mRNA and protein in lung cancer tissues was significantly upregulated (all P£¼0.05).There was a suppression of clonal formation and proliferation in A549 and H1299 cells in vitro and in vivo by inhibiting ILF2 expression (P£¼0.05).The expression of Ki67 in mice lung tissues was significantly reduced (P£¼0.05),and the levels of mtDNA and mitochondrial mass were significantly reduced,and the mitochondrial membrane potential was significantly increased in A549 and H1299 cells (all P£¼0.05).There was a increased capability of clonal formation and proliferation and mtDNA and mitochondrial mass,an decreased level of mitochondrial membrane potential in A549 and H1299 cells after ILF2 overexpressed (all P£¼0.05).Conclusion:ILF2 plays an important role in non-small cell lung cancer progression,which may be related to the destruction of mitochondrial homeostasis.

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Memo

Memo:

National Natural Science Foundation of China(No.81602026£©£»¹ú¼Ò×ÔÈ»¿Æѧ»ù½ð×ÊÖúÏîÄ¿£¨±àºÅ£º81602026£©£»Ìì½òÊÐÁÙ´²Öصãѧ¿Æ£¨×¨¿Æ£©½¨ÉèÏîÄ¿£¨±àºÅ£ºTJYXZDXK-047A£©

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