|Table of Contents|

Preliminary study on the effect of DNAJC2 on cells biological function in hepatocellular carcinoma

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 08
Page:
1375-1381
Research Field:
Publishing date:

Info

Title:
Preliminary study on the effect of DNAJC2 on cells biological function in hepatocellular carcinoma
Author(s):
XU Ziyun1; FAN Yuchun2; WEI Wanshuo3; ZHANG Chaosheng1; ZHANG Linli3; WU Tong3; JIANG Lihe1; 2
1.School of Basic Medicine,Youjiang Medical College for Nationalities,Guangxi Baise 533000,China;2.Medical College of Guangxi University,Guangxi Nanning 530004,China;3.School of Clinical Medicine,Youjiang Medical College for Nationalities,Guangxi Baise 533000,China.
Keywords:
hepatocellular carcinoma; DNAJC2; bioinformatics analysis; cell experiments
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2024.08.001
Abstract:
Objective:To explore the role of DNAJC2 in hepatocellular carcinoma and its effects on proliferation,migration,invasion and apoptosis of hepatocellular carcinoma cells.Methods:Based on bioinformatics analysis,the expression and prognostic role of DNAJC2 in hepatocellular carcinoma were investigated.The KEGG enrichment analysis was conducted on DNAJC2' s positive and negative correlated genes using TCGA database.The GDSC database was used to analyze drug sensitivity based on DNAJC2 expression.CCK-8 assay and colony formation assay,Transwell and scratch assay,Hoechst and JC-1 assay were performed to assess the effects of DNAJC2 knockdown on hepatocellular carcinoma MHCC97H cell proliferation,migration,invasion and apoptosis.Results:DNAJC2 was highly expressed in hepatocellular carcinoma and significantly correlated with patient prognosis.KEGG pathway enrichment analysis revealed that positively correlated genes of DNAJC2 were mainly enriched in pathways related to nucleocytoplasmic transport,and ubiquitin-mediated protein degradation,while negatively correlated genes were associated with complement and coagulation cascades.The high expression group of DNAJC2 showed higher sensitivity to sorafenib and sunitinib.Cell experiments showed that knockdown of DNAJC2 significantly reduced the proliferation,migration,and invasion,and promoted apoptosis of MHCC97H cells.Conclusion:DNAJC2 is highly expressed in hepatocellular carcinoma and has a favorable prognostic value.High levels of DNAJC2 are associated with increased sensitivity to sorafenib.In hepatocellular carcinoma,DNAJC2 may be involved in signaling pathways related to nucleocytoplasmic transport,ubiquitin-mediated protein degradation,complement and coagulation cascades.Knockdown of DNAJC2 can suppress the malignant behavior of hepatocellular carcinoma cells.

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2022年国家级大学生创新创业训练计划项目（编号：202210599007，202210599005）；广西医学遗传与基因组学研究重点实验室开放课题（编号：GXKMGG202201）；广西南宁市青秀区重点研发项目（编号：2020023）；右江民族医学院高层次引进人才项目（编号：yy2021sk002）
Last Update: 1900-01-01