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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2024 06

Page:

1075-1083

Research Field:

Publishing date:

Info

Title:

The expression and clinical significance of tiRNA-1_33-Gly-GCC-1 in colorectal adenocarcinoma tissue

Author(s):

ZHAI Chen¹; 2; WANG Huiyue¹; YAN Xuebing³; WANG Chenghai¹

1.Department of Pathology,School of Medicine,Yangzhou University,Jiangsu Yangzhou 225009,China;2.Danyang Third People's Hospital,Jiangsu Zhenjiang 212300,China;3.Department of Oncology,Affiliated Hospital of Yangzhou University,Jiangsu Yangzhou 225009,China.

Keywords:

colorectal adenocarcinoma; tiRNA-1_33-Gly-GCC-1; TP53I11; cell proliferation

PACS:

R735.3

DOI:

10.3969/j.issn.1672-4992.2024.06.016

Abstract:

Objective:To study the expression of tiRNA-1_33-Gly-GCC-1 in colorectal adenocarcinoma (CAC) tissues and its impact on cell proliferation.Methods:Expression profiles of tRFs and tiRNAs in 5 pairs of CAC tissues were detected and analyzed using Arraystar nrStarTM tRNA PCR Arrays,and the expression levels of tiRNA-1_33-Gly-GCC-1 in 82 pairs of CAC tissues were verified using qRT-PCR assay.RNA in situ hybridization (RISH) was used to detect the positive expression of tiRNA-1_33-Gly-GCC-1 in 82 CAC tissues.The correlation was statistically analyzed between its positive expression and the clinical pathological characteristics and prognosis of patients.CCK-8 experiment was used to detect the effect of tiRNA-1_33-Gly-GCC-1 on the proliferation activity of colorectal cancer cells.Bioinformatics and double luciferase report experiment were used to verify how tiRNA-1_33-Gly-GCC-1 regulates TP53I11.Rescue experiment to verify the effect of the tiRNA-1_33-Gly-GCC-1/TP53I11 signal axis on the proliferation of colorectal cancer cells.The subcutaneous transplanted tumors experiment in nude mice was used to verify the effect of tiRNA-1_33-Gly-GCC-1 on the growth and proliferation of transplanted tumors.Results:The expression of tiRNA-1_33-Gly-GCC-1 was increased and mainly positive in CAC tissues,with a significantly higher number of positive cases in 54/82 (65.85%) compared to 11/82 (13.41%) in adjacent normal tissues(χ2=5.475,P=0.019＜0.05).Positive expression of tiRNA-1_33-Gly-GCC-1 and tumor diameter in CAC patients（χ2=11.699)，degree of differentiation（χ2=13.081) and patient survival prognosis（χ2=15.621) is closely related (all P＜0.05).The results of the CCK-8 experiment showed tiRNA-1_33-Gly-GCC-1 increased the proliferative activity of CAC cells(P＜0.05).Bioinformatics predicts that TP53I11 is one of the downstream target genes of tiRNA-1_33-Gly-GCC-1.The double luciferase reporter experiment suggested that tiRNA-1_33-Gly-GCC-1 could binds to TP53I11 3' UTR to inhibit its protein synthesis.The Rescue experiment result further validated the inhibitory effect of tiRNA-1_33-Gly-GCC-1 on the expression of TP53I11 mRNA and protein and its promoting effect on the proliferation of colorectal cancer cells.The subcutaneous tumor transplantation experiment in nude mice also showed that tiRNA-1_33-Gly-GCC-1 promotes growth and proliferation.Conclusion:tiRNA-1_33-Gly-GCC-1 is upregulated in colorectal adenocarcinoma tissue and may be a new oncogenic gene.Mechanically,tiRNA-1_33-Gly-GCC-1 can inhibit the expression of TP53I11 and promote the growth and proliferation of CAC cells.This study provides a new theoretical basis for the diagnosis,treatment,and prognosis of CAC in the future.

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Memo

Memo:

江苏省卫生健康委科研项目(编号:M2020024)

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