|Table of Contents|

Study on serum proteomics of nasopharyngeal carcinoma patients with distant metastasis after radical treatment by data independent acquisition

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 05
Page:
833-838
Research Field:
Publishing date:

Info

Title:
Study on serum proteomics of nasopharyngeal carcinoma patients with distant metastasis after radical treatment by data independent acquisition
Author(s):
PENG Lingqipo; ZHUANG Jiafeng; JIANG Danxian; YANG Liu; HUANG Jing
Department of Head and Neck Oncology,Affiliated Hospital of Guangdong Medical University,Guangdong Zhanjiang 524001,China.
Keywords:
nasopharyngeal carcinoma; metastasis; proteomics; DIA
PACS:
R739.62
DOI:
10.3969/j.issn.1672-4992.2024.05.009
Abstract:
Objective:To investigate the differences in serum protein expression profiles between NPC patients with distant metastasis(rM) and those without metastasis(CR) after radical treatment.Methods:Serum samples were collected from five NPC patients who developed metastasis after radical treatment,and other five samples from those without metastasis.Data-independent acquisition(DIA) was used to compare the serum protein expression profiles of the two groups of patients,and differentially expressed proteins were identified.Enrichment analysis of the differentially expressed proteins was performed using gene ontology(GO) functional and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway analysis methods.Results:A total of 2 485 proteins were identified in all tested serum samples,and there were altogether 68 differentially expressed proteins were obtained through screening.Among them,61 were upregulated and 7 were downregulated in the metastatic group.Enrichment analysis showed that these differentially expressed proteins were mainly enriched in biological processes such as complement activation and immune regulation,and are closely related to molecular functions such as complement binding and multiple enzyme activities,and the cellular components such as blood particles,extracellular region,extracellular space and exosome.They were participated in key signal pathways such as complement and coagulation cascade,actin cytoskeleton regulation and cell apoptosis.Conclusion:Differentially expressed serum proteins may be closely related to NPC metastasis and have the potential to become potential prognostic indicators and intervention targets for metastatic NPC.

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广东省教育厅创新强校项目(编号：2014KQNCX095)
Last Update: 2024-01-30