|Table of Contents|

Exploring the anti-tumor mechanism of traditional Chinese medicine bufalin in osteosarcoma based on network pharmacology

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 04
Page:
629-635
Research Field:
Publishing date:

Info

Title:
Exploring the anti-tumor mechanism of traditional Chinese medicine bufalin in osteosarcoma based on network pharmacology
Author(s):
FANG Bo1; DING Xiaolei2
1.Department of Orthopaedic Surgery,Dalian Second People's Hospital,Liaoning Dalian 116011,China;2.Department of Oncology,Second Affiliated Hospital of Dalian Medical University,Liaoning Dalian 116023,China.
Keywords:
bufalin; network pharmacology; osteosarcoma; antitumor; mechanism
PACS:
R738.1
DOI:
10.3969/j.issn.1672-4992.2024.04.007
Abstract:
Objective:In this study,the network pharmacological analysis method was used to understand the anti-tumor mechanism of bufalin in osteosarcoma,and further verified by relevant experiments.Methods:The potential targets of bufalin were predicted through the PharmMapper database.The targets of osteosarcoma related diseases were searched through the GeneCards database.The targets in the two databases were analyzed with Venny.Then the String database was used to draw the protein interaction (PPI) network,and the David database was used to enrich the gene ontology (GO) and analyze the KEGG signal pathway of the key targets.Finally,Cytoscape 3.7.2 and Origin 2020 software were used to construct the network diagram of "compound-pathway-target-disease".Finally,CCK-8 experiment and Western blot experiment were used to verify.Results:A total of 107 common targets of bufalin and osteosarcoma were obtained through pharmacological analysis,and 34 related signal pathways were obtained through KEGG pathway enrichment analysis,involving PI3K-AKT signal pathway,MAPK signal pathway,p53 signal pathway,VEGF signal pathway,ErbB signal pathway,etc.The PPI network analysis showed that MYC,CCND1,IL6,ESR1,CDKN2A,IGF1,CAT,CDKN1A,ANXA5 and PTGS2 were the targets with the highest connectivity and weight.CCK-8 proved that bufalin could inhibit the proliferation of osteosarcoma cells.Western blot showed the expression of proliferation related proteins MYC and CCND1 was decreased.The recovery experiment further confirmed that bufalin may play an anti-osteosarcoma role by regulating PI3K-AKT signaling pathway.Conclusion:Based on the network pharmacological analysis and related experiments,it was found that bufalin may inhibit the growth of osteosarcoma by regulating PI3K-AKT signal transduction pathway.

References:

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