|Table of Contents|

The mechanism of Tie-2 in the process of oral squamous cell carcinoma invading the jaw

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 13
Page:
2412-2417
Research Field:
Publishing date:

Info

Title:
The mechanism of Tie-2 in the process of oral squamous cell carcinoma invading the jaw
Author(s):
AI Mengsheng1; SHEN Yang1; GAO Mingyu1; ZHANG Qinghua1; FEI Wei1; 2
1.School of Stomatology,Southwest Medical University,Sichuan Luzhou 646000,China;2.Department of Stomatology,Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital,Sichuan Chengdu 610072,China.
Keywords:
oral squamous cell carcinoma; Tie-2; osteoclasts; bone invasion
PACS:
R739.8
DOI:
10.3969/j.issn.1672-4992.2023.13.008
Abstract:
Objective:To study the mechanism of Tie-2(tyrosinekinase receptor-2) in the process of oral squamous cell carcinoma invading the jaw.Methods:Immunohistochemistry was used to compare the expression of Tie-2 in oral squamous cell carcinoma at the front of jaw invasion and other oral squamous cell carcinoma.Three siRNA lentiviral vectors(662,663,664) against Tie-2 were constructed as the experimental group,no-load virus 429 and normal saline（NS) as the control group,and each group was transfected into the hunan tongue squamous carcinoma cell line(Cal-27),the siRNA with the best inhibitory effect was screened by qRT-PCR.The cell viability was detected by CCK-8 method.The lentiviral vector with the best inhibitory effect was transfected into Cal-27,the culture supernatant was added to the human monocytic cell line(THP-1) medium,the expression of mRNA and protein in osteoclast function related genes TRAP(tartrate resistant acid phosphatase) and CTSL(cathepsin K) in THP-1 were detected by qRT-PCR and Western blotting.Results:The staining of the parosteal OSCC tissue at the invasion edge of the jaw was significantly deeper than that at other parts of the OSCC tissue，and the MOD value of parosteal OSCC tissue was significantly increased(P＜0.000 1).siRNA-664 had the best inhibitory effect on the expression of Tie-2 in Cal-27.After transfecting Cal-27 and THP-1 with lentivirus,the cell viability did not change significantly,and the difference was not statistically significant.After silencing the expression of Tie-2 in Cal-27,theosteoclast function-related genes and proteins of THP-1 significantly reduced expression.Conclusion:After down-regulating the expression of Tie-2,the potential of THP-1 to differentiate into osteoclasts was reduced,which is manifested as the down-regulation of osteoclast function-related genes and proteins.OSCC may release some osteoclast differentiation factors by activating the Ang/Tie-2 signaling system to promote osteoclast differentiation,thereby leading to local bone invasion.

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Memo

Memo:
四川省科技计划项目（编号：2017JY0082）；四川省人民医院院基金重点项目（编号：2021LZ01）
Last Update: 2023-05-31