|Table of Contents|

Effect of overexpression of lnc-TNFAIP3 gene on biological behavior of pancreatic cancer PANC-1 cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 13
Page:
2389-2394
Research Field:
Publishing date:

Info

Title:
Effect of overexpression of lnc-TNFAIP3 gene on biological behavior of pancreatic cancer PANC-1 cells
Author(s):
HU Xianhua1; ZHANG Rendan2; YAO Jiaxin1; ZHAO Chunyan3; MU Bo4
1.Department of Laboratory Medicine;2.Department of Stomatology;3.Key Laboratory of Medical Imaging;4.School of Basic Medicine and Forensic Medicine，North Sichuan Medical College,Sichuan Nanchong 637000,China.
Keywords:
lentivirus; transfection; lnc-TNFAIP3; PANC-1; pancreatic cancer
PACS:
R735.9
DOI:
10.3969/j.issn.1672-4992.2023.13.004
Abstract:
Objective:To construct pancreatic cancer cell lines with lnc-TNFAIP3 gene overexpression by lentivirus transfection and observe the changes of cell behavior.Methods:The overexpressed plasmid and lentivirus were prepared and transfected into human pancreatic cancer cell line PANC-1.The cell proliferation was detected by CCK-8 and cloning assay,the migration and invasion were detected by scratch and Transwell，the protein expression was detected by Western blot,and the cell cycle and apoptosis were detected by flow cytometry.Results:PANC-1 were successfully transfected with lentivirus overexpressing lnc-TNFAIP3 gene,and the proliferation,migration and invasion of PANC-1 were decreased.There was no significant difference in cell cycle and apoptosis,and no significant difference in the expression of related proteins.Conclusion:Human pancreatic cancer cell line PANC-1 constructed by lentivirus transfection with lnc-TNFAIP3 gene overexpression has changed in cell behavior,the present results suggest that lnc-TNFAIP3 may be a promising therapeutic target for pancreatic cancer,which provide a theoretical and experimental data for the study of lnc-TNFAIP3 gene in pancreatic cancer.

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Memo

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四川省科技创新（苗子工程）培育项目（编号：2021033）;四川省南充市校合作科研项目（编号：22SXCXTD0002，22SXQT0100)
Last Update: 2023-05-31