|Table of Contents|

Expression of LAMC3 in lung adenocarcinoma and its influence on cell biological behavior

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 02
Page:
257-263
Research Field:
Publishing date:

Info

Title:
Expression of LAMC3 in lung adenocarcinoma and its influence on cell biological behavior
Author(s):
WANG Lei1; 2; WAN Yunhui1; 2; YANG Jianping1; 2; SHI Tiansheng3
1.Jiangxi Health Vocational College,Jiangxi Nanchang 330052,China;2.Nanchang Medical College,Jiangxi Nanchang 330052,China;3.Department of Thoracic Surgery,Jiangxi Chest Hospital & The Third People's Hospital of Jiangxi,Jiangxi Nanchang 330006,China.
Keywords:
lung adenocarcinoma; laminin subunit gamma 3; cell proliferation; invasion and metastasis; epithelial-mesenchymal transition
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2023.02.011
Abstract:
Objective:To explore the expression of laminin subunit gamma 3(LAMC3) in lung adenocarcinoma(LUAD) and its influence on cell biological behavior.Methods:The online bioinformatics database GEPIA was used to query the expression of LAMC3 in LUAD tissues.The LUAD tissues and adjacent tissues were collected from 48 LUAD patients who underwent LUAD surgery in our hospital from January 2018 to December 2020,and RT-qPCR was used to detect the mRNA expression level of LAMC3 in the tissues,and the correlation between the expression level of LAMC3 and the clinicopathological characteristics of LUAD patients was analyzed.Human LUAD cell line(A549,H1299,PC-9) and human normal lung cell line MRC-5 were cultured in vitro,and RT-qPCR and Western blot were used to detect the mRNA and protein expression levels of LAMC3 in the cells.PC-9 cells were divided into five groups:Control group,si-NC group,LAMC3 silence group,pcDNA3.1-NC group and LAMC3 overexpression group.The small molecule RNA interference technology(siRNA) and pcDNA3.1 plasmid transfection were used to construct LAMC3 silence/overexpression cells.RT-qPCR and Western blot were used to detect the mRNA and protein expression levels of LAMC3 in each group of cells.CCK-8 method was used to detect cell proliferation activity in each group.Flow cytometry was used to detect the apoptosis rate of each group.Transwell chamber experiment was used to detect the migration and invasion abilities of each group of cells,and Western blot was used to detect the expression of epithelial-mesenchymal transition(EMT) related proteins［E-cadherin,N-cadherin,vimentin(VIM),matrix metalloproteinase 9(MMP-9)］of cells in each group.Results:LAMC3 was lowly expressed in LUAD tissues and cells,and the low expression of LAMC3 was significantly correlated with tumor size,TNM stage and lymph node metastasis(P<0.05).After silencing LAMC3,the abilities of cell proliferation,migration and invasion,and the expression of N-cadherin,VIM and MMP-9 increased,the apoptosis rate and the expression of E-cadherin decreased(P<0.05).After overexpression of LAMC3,the abilities of cell proliferation,migration and invasion decreased,the cell apoptosis rate increased(P<0.05),and the expression of E-cadherin in the cells increased,the expression of N-cadherin,VIM and MMP-9 decreased(P<0.05).Conclusion:LAMC3 plays an important role in the occurrence and development of LUAD.Overexpression of LAMC3 can inhibit the proliferation,migration and invasion of LUAD cells,and induce cell apoptosis.Its mechanism may be related to the inhibition of EMT.

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江西省教育厅科学技术研究项目（编号：GJJ191408）
Last Update: 1900-01-01