|Table of Contents|

Effect of arctigenin on drug resistance in ovarian cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 01
Page:
38-42
Research Field:
Publishing date:

Info

Title:
Effect of arctigenin on drug resistance in ovarian cancer cells
Author(s):
QI Chunlei; CAI Guoqing; GUO Qian; XU Jia
Department of Obstetrics and Gynecology,the First Affiliated Hospital of Air Force Medical University,Shaanxi Xi'an 710032,China.
Keywords:
arctigenin; drug resistance in ovarian cancer; SKOV3 cell; cisplatin; Survivin
PACS:
R737.31
DOI:
10.3969/j.issn.1672-4992.2023.01.006
Abstract:
Objective:To explore the effect and underlying mechanism of arctigenin on drug resistance of human ovarian cancer cisplatin resistant cell line SKOV3/DDP.Methods:Establish ovarian cancer drug resistant cell line SKOV3/DDP.SKOV3 and SKOV3/DDP were treated with arctigenin at different concentration (1,5,10,15,20,25 mmol/L).CCK-8 method was used to measure cell proliferation and screen optimum drug concentration.Then SKOV3/DDP were treated with 10 mmol/L arctigenin.Cell apoptosis was measured by flow cytometry.Protein expression of Caspase-3/9 and Cleaved Caspase-3/9 were measured by Western blot.IC50 were measured by CCK-8 method.mRNA and protein expression of Survivin were measured by RT-PCR and Western blot,respectively.SKOV3/DDP cell were treated with arctigenin and transfected with Survivin overexpression plasmid,then IC50 and cell apopotsis were texted.Results:Different concentrations of arctigenin inhibited cell growth of both SKOV3 and SKOV3/DDP.The inhibitory effect of arctigenin was higher in SKOV3/DDP than SKOV3 cells when the concentration of arctigenin ≥10 mmol/L (P＜0.05).10 mmol/L arctigenin treatment significantly increased SKOV3/DDP cell apoptosis,elevated protein expression of Caspase-3/9 and Cleaved Caspase-3/9,decreased DDP IC50,and inhibited mRNA and protein expression of Survivin (P＜0.05).After transfection of Survivin overexpression vector,IC50 was elevated and cell apoptosis was decreased in arctigenin treated SKOV3/DDP cells (P＜0.05).Conclusion:Arctigenin might inhibited drug resistance of SKOV3/DDP cells through suppressing Survivin.

References:

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Memo

Memo:
陕西省重点研发计划项目（编号：2019SF-165）;陕西省卫生健康科研基金项目（编号：2018D068）
Last Update: 2022-11-30