|Table of Contents|

The expression of miR-23b-3p in colorectal cancer and the mechanism of targeting KLF3 to inhibit the invasion and migration of colorectal cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 01
Page:
11-16
Research Field:
Publishing date:

Info

Title:
The expression of miR-23b-3p in colorectal cancer and the mechanism of targeting KLF3 to inhibit the invasion and migration of colorectal cancer cells
Author(s):
WANG Jinlin1; ZHAO Weifeng2; WANG Chaojie2
1.Department of Clinical Laboratory;2.Department of Oncology,Henan Provincial People's Hospital,Henan Zhengzhou 450003,China.
Keywords:
colorectal cancer; miR-23b-3p; Krüppel-like factor 3; invasion; migration
PACS:
R735.3
DOI:
10.3969/j.issn.1672-4992.2023.01.002
Abstract:
Objective:To investigate the expression of miR-23b-3p in colorectal cancer (CRC) and its effect on the invasion and migration of SW620 cells.Methods:58 cases of CRC tissue and para-cancerous tissue specimens were collected from January 2018 to December 2020 in gastrointestinal surgery in our hospital,in addition,CRC cell lines (SW480,SW620,HT-29 and Lovo) and human normal colorectal mucosal cells (FHC) were also chosen for this study.The relative expression level of miR-23b-3p and KLF3 was detected by quantitative polymerase chain reaction (qPCR).Transwell assay and wound healing assay were used to observe the invasion and migration of SW620 cells which was transfected with miR-23b-3p mimics or mimics-NC.The binding sites of miR-23b-3p and KLF3 were predicted by bioinformatics software and verified by double luciferase reporter gene assay.pcDNA3.1-KLF3 or vector was transfected into SW620 cells alone or in combination with miR-23b-3p mimics,transwell assay and wound healing assay were used to detected the invasion and migration of SW620 cells,the expression of KLF3 protein and mRNA in SW620 cells was detected by Western Blot and qPCR,respectively.Results:The expression of miR-23b-3p in CRC tissues was significantly lower than that in adjacent tissues (P＜0.05),which was related to the TNM stage and distant metastasis of CRC patients (all P＜0.05).The expression of miR-23b-3p in CRC cell lines was significantly lower than that in FHC cells,and up-regulation of miR-23b-3p expression could significantly inhibit the invasion and migration of SW620 cells.KLF3 was highly expressed in CRC tissues and cells,which was negatively correlated with miR-23b-3p expression in CRC tissues (r=-0.326,P=0.013),dual luciferase reporter gene assay confirmed that miR-23b-3p directly regulates KLF3 expression.Up-regulation of KLF3 can promote the invasion and migration of SW620 cells,meanwhile,transfection with miR-23b-3p mimics can down-regulate the expression of KLF3 protein and mRNA,reverse the promoting effection of pcDNA3.1-KLF3 on invasion and migration of SW620 cells.Conclusion:miR-23b-3p is low expressed in colorectal cancer and is associated with TNM stage and distant metastasis.miR-23b-3p inhibits the invasion and migration of SW620 cells by targeting KLF3.

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Last Update: 2022-11-30