|Table of Contents|

A prognostic model of gastric cancer based on RNA binding protein and its validation

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2022 04
Page:
652-659
Research Field:
Publishing date:

Info

Title:
A prognostic model of gastric cancer based on RNA binding protein and its validation
Author(s):
MA Pingchuan1; CHENG Guanghua1; DAI Yunhai1; TAO Shaoneng1; CHU Zhaoyang2
1.Nuclear Medicine Department;2.Ultrasound Medicine Department,Yijishan Hospital,Wannan Medical College,Anhui Wuhu 241000,China.
Keywords:
gastric cancer; RNA binding protein; overall survival rate; prognostic model; bioinformatics
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2022.04.019
Abstract:
Objective:To explore the prognostic model of gastric cancer with RNA binding protein and verify its accuracy by using bioinformatics.Methods:The RNA sequencing data of gastric cancer were downloaded from the Cancer Genome Atlas(TCGA) database,and the RBPs expressed in normal and tumor tissues were determined.The expression and prognostic value of these RBPs were studied systematically by bioinformatics analysis.Results:A total of 296 expressions of RBPs were identified,166 of which were up-regulated and 130 of which were down-regulated.Seven RBPs(RNASE1,SETD7,BOLL,ADARB1,PPARGC1B,LARP6,MSI2) were identified as prognostic hub genes.The prognostic model based on the above genes showed that the overall survival(OS) of the high-risk subgroup was lower than that of the low-risk subgroup.In the train group,the area under the ROC curve of the prognosis model was 0.730,while in the test group,it was 0.653.The expression differences of 7 RBPs in gastric cancer and normal tissue were further verified by the nomogram and HPA database.Conclusion:The RNA-binding protein-related prognostic model of gastric cancer shows a good prognostic differentiation ability,which will help to reveal the pathogenesis of gastric cancer and provide new clinical diagnosis and treatment ideas.

References:

[1]F BRAY,FERLAY J,SOERJOMATARAM I,et al.Global cancer statistics 2018:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries［J］.CA Cancer J Clin,2018,68(6):394-424.
[2]CHEN J,REN G,CAI R,et al.Cellular magnetic resonance imaging:in vivo tracking of gastric cancer cells and detecting of lymph node metastases using microparticles of iron oxide in mice［J］.Cancer Manag Res,2019,11:7317-7326.
[3]Q SUN,LI J,LI F,et al.LncRNA LOXL1-AS1 facilitates the tumorigenesis and stemness of gastric carcinoma via regulation of miR-708-5p/USF1 pathway［J］.Cell Prolif,2019,52(6):e12687.
[4]S GERSTBERGER,HAFNER M,TUSCHL T.A census of human RNA-binding proteins［J］.Nat Rev Genet,2014,15(12):829-845.
[5]K MASUDA,KUWANO Y.Diverse roles of RNA-binding proteins in cancer traits and their implications in gastrointestinal cancers［J］.Wiley Interdiscip Rev RNA,2019,10(3):e1520.
[6]N LEGRAND,DIXON DA,SOBOLEWSKI C.AU-rich element-binding proteins in colorectal cancer［J］.World J Gastrointest Oncol,2019,11(2):71-90.
[7]P CHATTERJI,RUSTGI AK.RNA binding proteins in intestinal epithelial biology and colorectal cancer［J］.Trends Mol Med,2018,24(5):490-506.
[8]B PEREIRA,BILLAUD M,ALMEIDA R.RNA-binding proteins in cancer:Old players and new actors［J］.Trends Cancer,2017,3(7):506-528.
[9]D SZKLARCZYK,GABLE AL,LYON D,et al.STRING v11:protein-protein association networks with increased coverage,supporting functional discovery in genome-wide experimental datasets［J］.Nucleic Acids Res,2019,47(D1):D607-D613.
[10]BALAZS GYORFFY,SUROWIAK PAWEL,BUDCZIES JAN,et al.Online survival analysis software to assess the prognostic value of biomarkers using transcriptomic data in non-small-cell lung cancer［J］.PloS One,2013,8(12):e82241.
[11]THUL PJ,AKESSON L,WIKING M,et al.A subcellular map of the human proteome［J］.Science,2017,356(6340):eaal3321.
[12]Y DUAN,DU A,GU J,et al.PARylation regulates stress granule dynamics,phase separation,and neurotoxicity of disease-related RNA-binding proteins［J］.Cell Res,2019,29(3):233-247.
[13]ECB JOHNSON,DAMMER EB,DUONG DM,et al.Deep proteomic network analysis of Alzheimer's disease brain reveals alterations in RNA binding proteins and RNA splicing associated with disease［J］.Mol Neurodegener,2018,13(1):52.
[14]RG DE BRUIN,RABELINK TJ,VAN ZONNEVELD AJ,et al.Emerging roles for RNA-binding proteins as effectors and regulators of cardiovascular disease［J］.Eur Heart J,2017,38(18):1380-1388.
[15]H CHEN,LIU J,WANG H,et al.Inhibition of RNA-binding protein Musashi-1 suppresses malignant properties and reverses paclitaxel resistance in ovarian carcinoma［J］.J Cancer,2019,10(6):1580-1592.
[16]H ZHANG,WANG Y,DOU J,et al.Acetylation of AGO2 promotes cancer progression by increasing oncogenic miR-19b biogenesis［J］.Oncogene,2019,38(9):1410-1431.
[17]FY ZONG,FU X,WEI WJ,et al.The RNA-binding protein QKI suppresses cancer-associated aberrant splicing［J］.PLoS Genet,2014,10(4):e1004289.
[18]HM JEONG,HAN J,LEE SH,et al.Correction:ESRP1 is overexpressed in ovarian cancer and promotes switching from mesenchymal to epithelial phenotype in ovarian cancer cells［J］.Oncogenesis,2019,8(9):47.
[19]WANG L,ZHU JS,SONG MQ,et al.Comparison of gene expression profiles between primary tumor and metastatic lesions in gastric cancer patients using laser microdissection and cDNA microarray［J］.World J Gastroenterol,2006,12(43):6949-6954.
[20]CHAN TH,QAMRA A,TAN KT,et al.ADAR-mediated RNA editing predicts progression and prognosis of gastric cancer［J］.Gastroenterology,2016,151(4):637-650.e10.
[21]YANG Z,LI J,SHI Y,et al.Increased musashi 2 expression indicates a poor prognosis and promotes malignant phenotypes in gastric cancer［J］.Oncol Lett,2019,17(3):2599-2606.

Memo

Memo:
安徽省中央引导地方科技发展专项项目（编号：2017070802D152）；皖南医学院重点科研项目培育基金（编号：WK2017ZF01）
Last Update: 1900-01-01