|Table of Contents|

The expression of ALMS1-IT1 in colorectal cancer and its effect on cancer cell proliferation,invasion and epithelial-mesenchymal transition

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2022 04
Page:
561-566
Research Field:
Publishing date:

Info

Title:
The expression of ALMS1-IT1 in colorectal cancer and its effect on cancer cell proliferation,invasion and epithelial-mesenchymal transition
Author(s):
WANG Haoyi1; 2; LIU Chang1; SUN Xuejun1
1.Department of General Surgery,the First Affiliated Hospital of Xi'an Jiaotong University,Shaanxi Xi'an 710061,China;2.Department of General Surgery,the First Hospital of Yulin,Shaanxi Yulin 718099,China.
Keywords:
ALMS1-IT1; colorectal cancer; proliferation; migration; invasion; EMT
PACS:
R735.3
DOI:
10.3969/j.issn.1672-4992.2022.04.001
Abstract:
Objective:To explore the expression and prognostic significance of lncRNA ALMS1-IT1 in colorectal cancer (CRC) and determine the impact of ALMS1-IT1 in CRC cell proliferation,invasion and epithelial-mesenchymal transition (EMT).Methods:The expression of ALMS1-IT1 in CRC and its correlation with the survival of patients were analyzed by GEPIA web server using The Cancer Genome Atlas (TCGA) database.The expression of ALMS1-IT was detected by RT-qPCR in human normal intestinal epithelial cells (NCM460) and CRC cells (HCT116,HT29,LoVo,SW480).ALMS1-IT was silenced and overexpressed by plasmid transfection in LoVo and SW480 cells,respectively.CCK-8 assay detected cell proliferation ability.Transwell assay analyzed cell migration and invasion potentials.Immunoblotting was used to detect the changes of EMT markers (E-cadherin,N-cadherin,Vimentin).Results:TCGA data analysis confirmed that ALMS1-IT expression in CRC tissues was significantly higher than that in normal tissues,and high expression of ALMS1-IT1 was prominently associated with poor prognosis.The expression of ALMS1-IT1 in CRC cells (HCT116,HT29,LoVo,SW480) was markedly higher than that in normal intestinal epithelial cells (NCM460).ALMS1-IT1 silencing inhibited cell proliferation,migration,invasion,and led to increased expression of E-cadherin protein and decreased expression of N-cadherin and Vimentin protein in LoVo cells.Overexpression of ALMS1-IT1 promoted cell proliferation,migration,invasion,and resulted in decreased expression of E-cadherin protein and increased expression of N-cadherin and Vimentin protein in SW480 cells.Conclusion:ALMS1-IT1 is highly expressed in CRC tissues and is closely related to poor prognosis.ALMS1-IT1 exerts an oncogenic role by promoting the proliferation,invasion and EMT of CRC cells.

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Memo

Memo:
National Natural Science Foundation of China(No.81972720）；国家自然科学基金资助项目（编号：81972720）
Last Update: 1900-01-01