|Table of Contents|

Molecular mechanism of miR-21 regulating potential target gene of pancreatic cancer and its clinical significance

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 06
Page:
979-984
Research Field:
Publishing date:

Info

Title:
Molecular mechanism of miR-21 regulating potential target gene of pancreatic cancer and its clinical significance
Author(s):
XIN Ruiqiang; WANG Shi; LI Shengli; HU Zhiwei; LIU Lianjie; ZHAO Lixin
Department of Hepatobiliary Surgery,Inner Mongolia People's Hospital,Inner Mongolia Hohhot 010017,China.
Keywords:
pancreatic ductal adenocarcinoma; miR-21; molecular mechanism
PACS:
R735.9
DOI:
10.3969/j.issn.1672-4992.2021.06.015
Abstract:
Objective:To confirm that miR-21 is an important target for regulating potential genes (PTEN,Bcl-2,c-Myc) of pancreatic ductal adenocarcinoma,and to analyze the expression and clinical significance of miR-21 in pancreatic ductal adenocarcinoma.Methods:The expression of miR-21,PTEN,Bcl-2 and c-Myc in pancreatic ductal adenocarcinoma and paracancerous pancreatic tissues was detected by Real-time PCR and immunohistochemistry.χ2 test was used to analyze the correlation between the expression of miR-21 and the expression levels of PTEN,Bcl-2 and c-Myc in pancreatic ductal adenocarcinoma and the correlation between the expression of miR-21 and the clinicopathological features of pancreatic cancer patients.Kaplan-Meier single factor analysis was used to calculate the cumulative survival rate and Log-rank test was used to analyze the relationship between the expression of miR-21 in pancreatic cancer tissue and the postoperative prognosis of pancreatic cancer patients.Results:The expression levels of miR-21,Bcl-2 and c-Myc in pancreatic ductal adenocarcinoma were significantly higher than those in the control group (P＜0.05).PTEN expression in pancreatic ductal adenocarcinoma was significantly lower than that in the control group (P＜0.05).There was a negative correlation between the expression of miR-21 and PTEN in pancreatic ductal adenocarcinoma,and a positive correlation between the expression of miR-21 and the expression of c-Myc and Bcl-2 in pancreatic ductal adenocarcinoma (P＜0.05).miR-21 expression was not related to gender,age,tumor differentiation,CA19-9 level and tumor location (P＞0.05),but related to tumor size,TNM stage and lymph node metastasis (P＜0.05).The survival rate of patients with high expression of miR-21 was significantly lower than that of patients with low expression of miR-21 (P＜0.05).Conclusion:miR-21 may be a key target in the regulation of PTEN,Bcl-2 and c-Myc in pancreatic ductal adenocarcinoma.miR-21 is highly expressed in pancreatic cancer tissue and is closely related to clinicopathological features,which can be used as an effective indicator for early diagnosis and prognosis.

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Memo

Memo:
内蒙古自治区自然科学基金项目（编号：2017MS08141）
Last Update: 1900-01-01